Information on EC 2.1.2.10 - aminomethyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY
2.1.2.10
-
RECOMMENDED NAME
GeneOntology No.
aminomethyltransferase
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
C-N bond cleavage
-
-
-
-
methyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
glycine cleavage
-
Glycine, serine and threonine metabolism
-
Metabolic pathways
-
One carbon pool by folate
-
SYSTEMATIC NAME
IUBMB Comments
[protein]-S8-aminomethyldihydrolipoyllysine:tetrahydrofolate aminomethyltransferase (ammonia-forming)
A component, with EC 1.4.4.2 glycine dehydrogenase (decarboxylating) and EC 1.8.1.4, dihydrolipoyl dehydrogenanse, of the glycine cleavage system, formerly known as glycine synthase. The glycine cleavage system is composed of four components that only loosely associate: the P protein (EC 1.4.4.2), the T protein (EC 2.1.2.10), the L protein (EC 1.8.1.4) and the lipoyl-bearing H protein [3].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
aminomethyltransferase
-
-
Amt
-
-
-
-
GCVT
-
two isogenes: GCVT-1 and GCVT-2
glycine synthase
-
-
-
-
synthase, glycine
-
-
-
-
T protein
-
-
-
-
T-protein
-
-
-
-
T-protein component of glycine cleavage complex
-
-
tetrahydrofolate aminomethyltransferase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37257-08-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
recombinant enzyme, expressed in Escherichia coli BL21 (DE3)pLysS
-
-
Manually annotated by BRENDA team
strain OT3
-
-
Manually annotated by BRENDA team
strain OT3, expression in Escherichia coli
-
-
Manually annotated by BRENDA team
Pyrococcus horikoshii OT-3
strain OT3
-
-
Manually annotated by BRENDA team
Pyrococcus horikoshii OT-3
strain OT3, expression in Escherichia coli
-
-
Manually annotated by BRENDA team
male Wistar rats
-
-
Manually annotated by BRENDA team
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0677
-
5,10-methylenetetrahydrofolate
-
-
0.0869
-
5,10-methylenetetrahydrofolate
-
mutant T4A, His-tagged
0.0875
-
5,10-methylenetetrahydrofolate
-
mutant L6A, His-tagged
0.0881
-
5,10-methylenetetrahydrofolate
-
wild-type with His-tag
0.151
-
5,10-methylenetetrahydrofolate
-
N-terminal deletion mutant, His-tagged
0.0092
-
5,10-methylenetetrahydropteroyltetraglutamate
-
wild-type with His-tag
0.0104
-
5,10-methylenetetrahydropteroyltetraglutamate
-
-
0.0108
-
5,10-methylenetetrahydropteroyltetraglutamate
-
mutant L6A, His-tagged
0.0174
-
5,10-methylenetetrahydropteroyltetraglutamate
-
mutant T4A, His-tagged
65.4
-
NH4Cl
-
wild-type with His-tag
78.9
-
NH4Cl
-
mutant L6A, His-tagged
93.5
-
NH4Cl
-
mutant T4A, His-tagged
256
-
NH4Cl
-
N-terminal deletion mutant, His-tagged
0.00069
-
reduced H-protein
-
wild-type with His-tag
-
0.0012
-
reduced H-protein
-
mutant T4A, His-tagged
-
0.00237
-
reduced H-protein
-
mutant L6A, His-tagged
-
0.0169
-
reduced H-protein
-
N-terminal deletion mutant, His-tagged
-
0.0037
-
S-Aminomethyldihydrolipoylprotein
-
-
0.17
-
tetrahydrofolate
-
-
0.0382
-
5,10-methylenetetrahydropteroyltetraglutamate
-
N-terminal deletion mutant, His-tagged
additional information
-
additional information
-
values for mutant enzymes
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2.1
-
5,10-methylenetetrahydrofolate
-
N-terminal deletion mutant, His-tagged
7.8
-
5,10-methylenetetrahydrofolate
-
mutant T4A, His-tagged
10.3
-
5,10-methylenetetrahydrofolate
-
mutant L6A, His-tagged
14.4
-
5,10-methylenetetrahydrofolate
-
-
18.4
-
5,10-methylenetetrahydrofolate
-
wild-type with His-tag
8.75
-
5,10-methylenetetrahydropteroyltetraglutamate
-
-
9.5
-
5,10-methylenetetrahydropteroyltetraglutamate
-
N-terminal deletion mutant, His-tagged
10.5
-
5,10-methylenetetrahydropteroyltetraglutamate
-
wild-type with His-tag
10.6
-
5,10-methylenetetrahydropteroyltetraglutamate
-
mutant L6A, His-tagged
3.9
-
NH4Cl
-
N-terminal deletion mutant, His-tagged
9.6
-
NH4Cl
-
mutant T4A, His-tagged
11.6
-
NH4Cl
-
mutant L6A, His-tagged
20.9
-
NH4Cl
-
wild-type with His-tag
2.9
-
reduced H-protein
-
N-terminal deletion mutant, His-tagged
-
8.5
-
reduced H-protein
-
mutant T4A, His-tagged
-
13.3
-
reduced H-protein
-
mutant L6A, His-tagged
-
19.4
-
reduced H-protein
-
wild-type with His-tag
-
11.6
-
5,10-methylenetetrahydropteroyltetraglutamate
-
mutant T4A, His-tagged
additional information
-
additional information
-
values for mutant enzymes
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
-
-
assay at
additional information
-
-
pI: 9.8
PDB
SCOP
CATH
ORGANISM
Bacillus subtilis (strain 168)
Bartonella henselae (strain ATCC 49882 / Houston 1)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
lability of enzyme under the conditions of its purifications and storage
-