General Stability | Organism |
---|---|
lability of enzyme under the conditions of its purifications and storage | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
S-aminomethyldihydrolipoylprotein | - |
Gallus gallus | |
0.17 | - |
tetrahydrofolate | - |
Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Gallus gallus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | 38000 | sedimentation equilibrium centrifugation, gel filtration | Gallus gallus |
41000 | - |
1 * 41000, SDS-PAGE | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate | Gallus gallus | part of the glycine cleavage system | dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
641fold purification | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Gallus gallus |
Storage Stability | Organism |
---|---|
-20°C, 20 mM Tris-HCl buffer, pH 8.0, 10 mM 2-mercaptoethanol, 50% v/v glycerol, at least 2 months, stable | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | component of the glycine cleavage system, T-protein is associated with H-protein forming a complex which is composed of one molecule of each of them | Gallus gallus | ? | - |
? | |
S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate | decarboxylated glycine moiety attached to H-protein + tetrahydrofolate as substrates | Gallus gallus | dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3 | - |
r | |
S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate | strictly dependent on tetrahydrofolate | Gallus gallus | dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3 | - |
r | |
S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate | part of the glycine cleavage system | Gallus gallus | dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, SDS-PAGE | Gallus gallus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 9.8 | Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrofolate | strictly dependent on tetrahydrofolate | Gallus gallus |