Information on EC 1.4.3.25 - L-arginine oxidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.4.3.25
-
RECOMMENDED NAME
GeneOntology No.
L-arginine oxidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-arginine + H2O + O2 = 5-guanidino-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-arginine degradation VIII (arginine oxidase pathway)
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Arginine and proline metabolism
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SYSTEMATIC NAME
IUBMB Comments
L-arginine:oxygen oxidoreductase (deaminating)
Contains FAD. The enzyme from cyanobacteria can also act on other basic amino acids with lower activity. The enzyme from the bacterium Pseudomonas sp. TPU 7192 is highly specific.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
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HW613270
GenBank
Manually annotated by BRENDA team
-
HW613270
GenBank
Manually annotated by BRENDA team
Synechococcus cedorum
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-
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Manually annotated by BRENDA team
Synechococcus cedorum PCC 6908
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-
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-arginine + H2O + O2
5-guanidino-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
L-canavanine + H2O + O2
2-oxocanavanine + NH3 + H2O2
show the reaction diagram
L-histidine + H2O + O2
2-oxohistidine + NH3 + H2O2
show the reaction diagram
L-homoarginine + H2O + O2
2-oxohomoarginine + NH3 + H2O2
show the reaction diagram
L-lysine + H2O + O2
2-oxolysine + NH3 + H2O2
show the reaction diagram
L-ornithine + H2O + O2
2-oxoornithine + NH3 + H2O2
show the reaction diagram
additional information
?
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enzyme catalyzes the oxidative deamination of the basic amino acids: L-arginine, L-lysine, L-ornithine and L-histidine. In the presence of catalase and of any of these amino acids, 0.5 mol O2 is consumed, and 1 mol ammonia is formed for each mol amino acid oxidized
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-arginine + H2O + O2
5-guanidino-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
nordihydroguaretic acid
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0.5 mM, 63% inhibition
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o-naphthol
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10 mM, 53% inhibition
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o-phenanthroline
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5 mM, 72% inhibition
sdodium azide
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100 mM, 77% inhibition
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additional information
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not inhibitory: EDTA, p-chloromercuric acid, hydroxylamine, p-aminobenzoic acid, 8-hydroxyquinoline, sodium cyanide
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.149 - 5
L-arginine
5.25
L-lysine
HW613270
pH 7.0, 37C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.1 - 1.6
Ca2+
0.15
Cd2+
Synechococcus elongatus PCC 7942
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pH 7.0, 20C
0.38
Co2+
Synechococcus elongatus PCC 7942
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pH 7.0, 20C
0.06
Cu2+
Synechococcus elongatus PCC 7942
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pH 7.0, 20C
0.16
Mn2+
Synechococcus elongatus PCC 7942
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pH 7.0, 20C
0.065
Zn2+
Synechococcus elongatus PCC 7942
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pH 7.0, 20C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
120.3
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substrate L-arginine, 20C, pH 7.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
HW613270
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6 - 6.5
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substrate L-histidine
7
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substrate L-arginine
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
HW613270
-
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
98000
-
gel filtration
528000
HW613270
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 49000, SDS-PAGE
octamer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 7.5
HW613270
-
740395
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
HW613270
stable up to
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
both native and recombinant protein
HW613270
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
-
HW613270