Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.3.25 extracted from

  • Pistorius, E.K.; Voss, H.
    Some properties of a basic L-amino-acid oxidase from Anacystis nidulans (1980), Biochim. Biophys. Acta, 611, 227-240.
    View publication on PubMed
Search for more literature for 1.4.3.25

Inhibitors

Inhibitors Comment Organism Structure
1-naphthol 10 mM, 53% inhibition Synechococcus elongatus PCC 7942 = FACHB-805
Cd2+
-
 Synechococcus elongatus PCC 7942 = FACHB-805
Co2+
-
 Synechococcus elongatus PCC 7942 = FACHB-805
Cu2+
-
 Synechococcus elongatus PCC 7942 = FACHB-805
Mn2+
-
 Synechococcus elongatus PCC 7942 = FACHB-805
additional information not inhibitory: EDTA, p-chloromercuric acid, hydroxylamine, p-aminobenzoic acid, 8-hydroxyquinoline, sodium cyanide Synechococcus elongatus PCC 7942 = FACHB-805
nordihydroguaiaretic acid 0.5 mM, 63% inhibition Synechococcus elongatus PCC 7942 = FACHB-805
o-phenanthroline 5 mM, 72% inhibition Synechococcus elongatus PCC 7942 = FACHB-805
Sodium azide 100 mM, 77% inhibition Synechococcus elongatus PCC 7942 = FACHB-805
Zn2+
-
 Synechococcus elongatus PCC 7942 = FACHB-805

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 2 * 49000, SDS-PAGE Synechococcus elongatus PCC 7942 = FACHB-805
98000
-
 gel filtration Synechococcus elongatus PCC 7942 = FACHB-805

Organism

Organism UniProt Comment Textmining
Synechococcus elongatus PCC 7942 = FACHB-805
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Synechococcus elongatus PCC 7942 = FACHB-805

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
120.3
-
 substrate L-arginine, 20°C, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O + O2
-
 Synechococcus elongatus PCC 7942 = FACHB-805 5-guanidino-2-oxopentanoate + NH3 + H2O2
-
 ?
L-histidine + H2O + O2
-
 Synechococcus elongatus PCC 7942 = FACHB-805 2-oxohistidine + NH3 + H2O2
-
 ?
L-lysine + H2O + O2
-
 Synechococcus elongatus PCC 7942 = FACHB-805 2-oxolysine + NH3 + H2O2
-
 ?
L-ornithine + H2O + O2
-
 Synechococcus elongatus PCC 7942 = FACHB-805 2-oxoornithine + NH3 + H2O2
-
 ?
additional information enzyme catalyzes the oxidative deamination of the basic amino acids: L-arginine, L-lysine, L-ornithine and L-histidine. In the presence of catalase and of any of these amino acids, 0.5 mol O2 is consumed, and 1 mol ammonia is formed for each mol amino acid oxidized Synechococcus elongatus PCC 7942 = FACHB-805 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 49000, SDS-PAGE Synechococcus elongatus PCC 7942 = FACHB-805

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 substrate L-histidine Synechococcus elongatus PCC 7942 = FACHB-805
7
-
 substrate L-arginine Synechococcus elongatus PCC 7942 = FACHB-805

Cofactor

Cofactor Comment Organism Structure
FAD 1 mol per mol of enzyme Synechococcus elongatus PCC 7942 = FACHB-805

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.06
-
 pH 7.0, 20°C Synechococcus elongatus PCC 7942 = FACHB-805 Cu2+
0.065
-
 pH 7.0, 20°C Synechococcus elongatus PCC 7942 = FACHB-805 Zn2+
0.15
-
 pH 7.0, 20°C Synechococcus elongatus PCC 7942 = FACHB-805 Cd2+
0.16
-
 pH 7.0, 20°C Synechococcus elongatus PCC 7942 = FACHB-805 Mn2+
0.38
-
 pH 7.0, 20°C Synechococcus elongatus PCC 7942 = FACHB-805 Co2+