Information on EC 1.1.1.16 - galactitol 2-dehydrogenase

Word Map on EC 1.1.1.16
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.1.16
-
RECOMMENDED NAME
GeneOntology No.
galactitol 2-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
galactitol + NAD+ = D-tagatose + NADH + H+
show the reaction diagram
Also converts other alditols containing an L-threo-configuration adjacent to a primary alcohol group into the corresponding sugars
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-altritol and galactitol degradation
-
-
Galactose metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
galactitol:NAD+ 2-oxidoreductase
Also converts other alditols containing an L-threo-configuration adjacent to a primary alcohol group into the corresponding sugars.
CAS REGISTRY NUMBER
COMMENTARY hide
9028-23-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene ladB
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene lad1
-
-
Manually annotated by BRENDA team
gene lad1
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-ribitol + NAD+
? + NADH
show the reaction diagram
-
-
-
?
galactitol + NAD+
D-tagatose + NADH
show the reaction diagram
-
-
-
r
galactitol + NAD+
L-xylo-3-hexulose + NADH + H+
show the reaction diagram
galactitol + NAD+
tagatose + NADH
show the reaction diagram
L-arabitol + NAD+
? + NADH + H+
show the reaction diagram
L-arabitol + NAD+
L-ribulose + NADH + H+
show the reaction diagram
-
-
-
?
L-arabitol + NAD+
L-xylulose + NADH + H+
show the reaction diagram
L-iditol + NAD+
L-sorbose + NADH
show the reaction diagram
-
-
-
r
sorbitol + NAD+
D-fructose + NADH
show the reaction diagram
xylitol + NAD+
? + NADH
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-ribitol + NAD+
? + NADH
show the reaction diagram
-
-
-
?
galactitol + NAD+
D-tagatose + NADH
show the reaction diagram
-
-
-
r
galactitol + NAD+
L-xylo-3-hexulose + NADH + H+
show the reaction diagram
galactitol + NAD+
tagatose + NADH
show the reaction diagram
L-arabitol + NAD+
L-ribulose + NADH + H+
show the reaction diagram
-
-
-
?
L-arabitol + NAD+
L-xylulose + NADH + H+
show the reaction diagram
L-iditol + NAD+
L-sorbose + NADH
show the reaction diagram
-
-
-
r
sorbitol + NAD+
D-fructose + NADH
show the reaction diagram
xylitol + NAD+
? + NADH
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
200
galactitol
1 - 55
L-arabitol
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 5% loss of activity within 5 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
recombinant C-terminally His6-tagged LadB from Saccharomyces cerevisiae strain CEN.PK2-1D by nickel affinity chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene lad1, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene ladB, orf JGI183900, DNA and amino acid sequence determination and analysis, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), functional expression of gene ladB under the lac promoter, functional co-expression of Aspergillus niger wild-type and C-terminally His6-tagged LadB and LadA, encoding L-arabitol dehydrogenae, in Saccharomyces cerevisiae strain CEN.PK2-1D
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
gene lad1 is induced in response to L-arabinose, D-galactose, and galactitol
gene ladB is induced in response to D-galactose and galactitol
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information