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Identification of the galactitol dehydrogenase, LadB, that is part of the oxido-reductive D-galactose catabolic pathway in Aspergillus niger

Mojzita, D.; Koivistoinen, O.M.; Maaheimo, H.; Penttilae, M.; Ruohonen, L.; Richard, P.; Fungal Genet. Biol. 49, 152-159 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene lad1, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Trichoderma reesei
gene ladB, orf JGI183900, DNA and amino acid sequence determination and analysis, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), functional expression of gene ladB under the lac promoter, functional co-expression of Aspergillus niger wild-type and C-terminally His6-tagged LadB and LadA, encoding L-arabitol dehydrogenae, in Saccharomyces cerevisiae strain CEN.PK2-1D
Aspergillus niger
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of ladBDELTA enzyme-deficient Aspergillus niger strains
Aspergillus niger
additional information
construction of ladBDELTA enzyme-deficient Aspergillus niger strains
Trichoderma reesei
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
-
L-arabitol
pH and temperature not specified in the publication
Trichoderma reesei
55
-
L-arabitol
pH and temperature not specified in the publication
Aspergillus niger
200
-
galactitol
about, pH and temperature not specified in the publication
Aspergillus niger
200
-
galactitol
about, pH and temperature not specified in the publication
Trichoderma reesei
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
galactitol + NAD+
Aspergillus niger
-
L-xylo-3-hexulose + NADH + H+
-
-
r
galactitol + NAD+
Trichoderma reesei
L-xylo-3-hexulose is a product of the Lad1 reaction in the alternative D-galactose pathway in Trichoderma reesei
L-xylo-3-hexulose + NADH + H+
-
-
r
galactitol + NAD+
Aspergillus niger ATCC 1015, CBS 113.46
-
L-xylo-3-hexulose + NADH + H+
-
-
r
galactitol + NAD+
Trichoderma reesei QM6a
L-xylo-3-hexulose is a product of the Lad1 reaction in the alternative D-galactose pathway in Trichoderma reesei
L-xylo-3-hexulose + NADH + H+
-
-
r
L-arabitol + NAD+
Trichoderma reesei
Lad1 is also active in the L-arabinose pathway as the L-arabitol-4-dehydrogenase
L-xylulose + NADH + H+
-
-
r
L-arabitol + NAD+
Trichoderma reesei QM6a
Lad1 is also active in the L-arabinose pathway as the L-arabitol-4-dehydrogenase
L-xylulose + NADH + H+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus niger
-
gene ladB
-
Aspergillus niger ATCC 1015, CBS 113.46
-
gene ladB
-
Trichoderma reesei
-
gene lad1
-
Trichoderma reesei QM6a
-
gene lad1
-
Purification (Commentary)
Commentary
Organism
recombinant C-terminally His6-tagged LadB from Saccharomyces cerevisiae strain CEN.PK2-1D by nickel affinity chromatography
Aspergillus niger
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
galactitol + NAD+
-
722320
Aspergillus niger
L-xylo-3-hexulose + NADH + H+
-
-
-
r
galactitol + NAD+
-
722320
Aspergillus niger
L-xylo-3-hexulose + NADH + H+
HPLC and NMR product analyses
-
-
r
galactitol + NAD+
-
722320
Trichoderma reesei
L-xylo-3-hexulose + NADH + H+
HPLC and NMR product analyses
-
-
r
galactitol + NAD+
L-xylo-3-hexulose is a product of the Lad1 reaction in the alternative D-galactose pathway in Trichoderma reesei
722320
Trichoderma reesei
L-xylo-3-hexulose + NADH + H+
-
-
-
r
galactitol + NAD+
-
722320
Aspergillus niger ATCC 1015, CBS 113.46
L-xylo-3-hexulose + NADH + H+
-
-
-
r
galactitol + NAD+
-
722320
Aspergillus niger ATCC 1015, CBS 113.46
L-xylo-3-hexulose + NADH + H+
HPLC and NMR product analyses
-
-
r
galactitol + NAD+
-
722320
Trichoderma reesei QM6a
L-xylo-3-hexulose + NADH + H+
HPLC and NMR product analyses
-
-
r
galactitol + NAD+
L-xylo-3-hexulose is a product of the Lad1 reaction in the alternative D-galactose pathway in Trichoderma reesei
722320
Trichoderma reesei QM6a
L-xylo-3-hexulose + NADH + H+
-
-
-
r
L-arabitol + NAD+
-
722320
Trichoderma reesei
L-xylulose + NADH + H+
-
-
-
r
L-arabitol + NAD+
Lad1 is also active in the L-arabinose pathway as the L-arabitol-4-dehydrogenase
722320
Trichoderma reesei
L-xylulose + NADH + H+
-
-
-
r
L-arabitol + NAD+
-
722320
Trichoderma reesei QM6a
L-xylulose + NADH + H+
-
-
-
r
L-arabitol + NAD+
Lad1 is also active in the L-arabinose pathway as the L-arabitol-4-dehydrogenase
722320
Trichoderma reesei QM6a
L-xylulose + NADH + H+
-
-
-
r
L-arabitol + NAD+
-
722320
Aspergillus niger
? + NADH + H+
-
-
-
r
L-arabitol + NAD+
-
722320
Aspergillus niger ATCC 1015, CBS 113.46
? + NADH + H+
-
-
-
r
additional information
the enzyme is also active with ribitol, xylitol, and to a lesser extent with D-sorbitol. No activity with mannitol and D-arabitol
722320
Aspergillus niger
?
-
-
-
-
additional information
the enzyme is also active with ribitol, xylitol, and to a lesser extent with D-sorbitol. No activity with mannitol and D-arabitol
722320
Trichoderma reesei
?
-
-
-
-
additional information
the enzyme is also active with ribitol, xylitol, and to a lesser extent with D-sorbitol. No activity with mannitol and D-arabitol
722320
Aspergillus niger ATCC 1015, CBS 113.46
?
-
-
-
-
additional information
the enzyme is also active with ribitol, xylitol, and to a lesser extent with D-sorbitol. No activity with mannitol and D-arabitol
722320
Trichoderma reesei QM6a
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
dependent on
Aspergillus niger
NAD+
dependent on
Trichoderma reesei
NADH
dependent on
Aspergillus niger
NADH
dependent on
Trichoderma reesei
Cloned(Commentary) (protein specific)
Commentary
Organism
gene lad1, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Trichoderma reesei
gene ladB, orf JGI183900, DNA and amino acid sequence determination and analysis, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), functional expression of gene ladB under the lac promoter, functional co-expression of Aspergillus niger wild-type and C-terminally His6-tagged LadB and LadA, encoding L-arabitol dehydrogenae, in Saccharomyces cerevisiae strain CEN.PK2-1D
Aspergillus niger
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
dependent on
Aspergillus niger
NAD+
dependent on
Trichoderma reesei
NADH
dependent on
Aspergillus niger
NADH
dependent on
Trichoderma reesei
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of ladBDELTA enzyme-deficient Aspergillus niger strains
Aspergillus niger
additional information
construction of ladBDELTA enzyme-deficient Aspergillus niger strains
Trichoderma reesei
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
-
L-arabitol
pH and temperature not specified in the publication
Trichoderma reesei
55
-
L-arabitol
pH and temperature not specified in the publication
Aspergillus niger
200
-
galactitol
about, pH and temperature not specified in the publication
Aspergillus niger
200
-
galactitol
about, pH and temperature not specified in the publication
Trichoderma reesei
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
galactitol + NAD+
Aspergillus niger
-
L-xylo-3-hexulose + NADH + H+
-
-
r
galactitol + NAD+
Trichoderma reesei
L-xylo-3-hexulose is a product of the Lad1 reaction in the alternative D-galactose pathway in Trichoderma reesei
L-xylo-3-hexulose + NADH + H+
-
-
r
galactitol + NAD+
Aspergillus niger ATCC 1015, CBS 113.46
-
L-xylo-3-hexulose + NADH + H+
-
-
r
galactitol + NAD+
Trichoderma reesei QM6a
L-xylo-3-hexulose is a product of the Lad1 reaction in the alternative D-galactose pathway in Trichoderma reesei
L-xylo-3-hexulose + NADH + H+
-
-
r
L-arabitol + NAD+
Trichoderma reesei
Lad1 is also active in the L-arabinose pathway as the L-arabitol-4-dehydrogenase
L-xylulose + NADH + H+
-
-
r
L-arabitol + NAD+
Trichoderma reesei QM6a
Lad1 is also active in the L-arabinose pathway as the L-arabitol-4-dehydrogenase
L-xylulose + NADH + H+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant C-terminally His6-tagged LadB from Saccharomyces cerevisiae strain CEN.PK2-1D by nickel affinity chromatography
Aspergillus niger
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
galactitol + NAD+
-
722320
Aspergillus niger
L-xylo-3-hexulose + NADH + H+
-
-
-
r
galactitol + NAD+
-
722320
Aspergillus niger
L-xylo-3-hexulose + NADH + H+
HPLC and NMR product analyses
-
-
r
galactitol + NAD+
-
722320
Trichoderma reesei
L-xylo-3-hexulose + NADH + H+
HPLC and NMR product analyses
-
-
r
galactitol + NAD+
L-xylo-3-hexulose is a product of the Lad1 reaction in the alternative D-galactose pathway in Trichoderma reesei
722320
Trichoderma reesei
L-xylo-3-hexulose + NADH + H+
-
-
-
r
galactitol + NAD+
-
722320
Aspergillus niger ATCC 1015, CBS 113.46
L-xylo-3-hexulose + NADH + H+
-
-
-
r
galactitol + NAD+
-
722320
Aspergillus niger ATCC 1015, CBS 113.46
L-xylo-3-hexulose + NADH + H+
HPLC and NMR product analyses
-
-
r
galactitol + NAD+
-
722320
Trichoderma reesei QM6a
L-xylo-3-hexulose + NADH + H+
HPLC and NMR product analyses
-
-
r
galactitol + NAD+
L-xylo-3-hexulose is a product of the Lad1 reaction in the alternative D-galactose pathway in Trichoderma reesei
722320
Trichoderma reesei QM6a
L-xylo-3-hexulose + NADH + H+
-
-
-
r
L-arabitol + NAD+
-
722320
Trichoderma reesei
L-xylulose + NADH + H+
-
-
-
r
L-arabitol + NAD+
Lad1 is also active in the L-arabinose pathway as the L-arabitol-4-dehydrogenase
722320
Trichoderma reesei
L-xylulose + NADH + H+
-
-
-
r
L-arabitol + NAD+
-
722320
Trichoderma reesei QM6a
L-xylulose + NADH + H+
-
-
-
r
L-arabitol + NAD+
Lad1 is also active in the L-arabinose pathway as the L-arabitol-4-dehydrogenase
722320
Trichoderma reesei QM6a
L-xylulose + NADH + H+
-
-
-
r
L-arabitol + NAD+
-
722320
Aspergillus niger
? + NADH + H+
-
-
-
r
L-arabitol + NAD+
-
722320
Aspergillus niger ATCC 1015, CBS 113.46
? + NADH + H+
-
-
-
r
additional information
the enzyme is also active with ribitol, xylitol, and to a lesser extent with D-sorbitol. No activity with mannitol and D-arabitol
722320
Aspergillus niger
?
-
-
-
-
additional information
the enzyme is also active with ribitol, xylitol, and to a lesser extent with D-sorbitol. No activity with mannitol and D-arabitol
722320
Trichoderma reesei
?
-
-
-
-
additional information
the enzyme is also active with ribitol, xylitol, and to a lesser extent with D-sorbitol. No activity with mannitol and D-arabitol
722320
Aspergillus niger ATCC 1015, CBS 113.46
?
-
-
-
-
additional information
the enzyme is also active with ribitol, xylitol, and to a lesser extent with D-sorbitol. No activity with mannitol and D-arabitol
722320
Trichoderma reesei QM6a
?
-
-
-
-
Expression
Organism
Commentary
Expression
Aspergillus niger
gene ladB is induced in response to D-galactose and galactitol
up
Trichoderma reesei
gene lad1 is induced in response to L-arabinose, D-galactose, and galactitol
up
General Information
General Information
Commentary
Organism
evolution
LadB belongs to the medium-chain dehydrogenases
Aspergillus niger
malfunction
deletion of ladB in Aspergillus niger results in growth arrest on galactitol and significantly slower growth on D-galactose supplemented with a small amount of D-xylose. D-Galactose alone cannot be utilised by Aspergillus niger and the addition of D-xylose stimulates growth on D-galactose via transcriptional activation of the D-xylose-inducible reductase gene, xyrA
Aspergillus niger
metabolism
LadB catalyzes a step in the oxido-reductive pathway for D-galactose catabolism in filamentous fungi, overview
Aspergillus niger
General Information (protein specific)
General Information
Commentary
Organism
evolution
LadB belongs to the medium-chain dehydrogenases
Aspergillus niger
malfunction
deletion of ladB in Aspergillus niger results in growth arrest on galactitol and significantly slower growth on D-galactose supplemented with a small amount of D-xylose. D-Galactose alone cannot be utilised by Aspergillus niger and the addition of D-xylose stimulates growth on D-galactose via transcriptional activation of the D-xylose-inducible reductase gene, xyrA
Aspergillus niger
metabolism
LadB catalyzes a step in the oxido-reductive pathway for D-galactose catabolism in filamentous fungi, overview
Aspergillus niger
Expression (protein specific)
Organism
Commentary
Expression
Aspergillus niger
gene ladB is induced in response to D-galactose and galactitol
up
Trichoderma reesei
gene lad1 is induced in response to L-arabinose, D-galactose, and galactitol
up
Other publictions for EC 1.1.1.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722320
Mojzita
Identification of the galactit ...
Aspergillus niger, Aspergillus niger ATCC 1015, CBS 113.46, Trichoderma reesei, Trichoderma reesei QM6a
Fungal Genet. Biol.
49
152-159
2012
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18
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4
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18
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3
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2
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287244
Allenza
Enzymes related to fructose ut ...
Burkholderia cepacia
J. Bacteriol.
150
1348-1356
1982
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389406
Primrose
Polyol metabolism by Rhizobium ...
Rhizobium leguminosarum
J. Bacteriol.
141
1109-1114
1980
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389407
Shaw
-
Galactitol dehydrogenase ...
Pseudomonas sp.
Methods Enzymol.
5
323-325
1962
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