6.3.2.34: coenzyme F420-1:gamma-L-glutamate ligase
This is an abbreviated version!
For detailed information about coenzyme F420-1:gamma-L-glutamate ligase, go to the full flat file.
Reaction
Synonyms
bifunctional F420 biosynthesis protein FbiB, BQ2027_MB3290, CofE, CofE-AF, F420-0:gamma-glutamyl ligase, F420:gamma-glutamyl ligase, FbiB, MJ0768
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6.3.2.34 coenzyme F420-1:gamma-L-glutamate ligaseAdvanced search results
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Metals Ions on EC 6.3.2.34 - coenzyme F420-1:gamma-L-glutamate ligase
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METALS and IONS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Co2+
divalent cation requirement, maximum CofE activity is observed with the addition of 10 mM MnCl2. Reactions containing 10 mM either MgCl2 or CoCl2 produce 40% of the F420-2 of that is produced with MnCl2
K+
CofE absolutely requires a monovalent cation for activity, the greatest extent of activation is achieved by K+, with maximum stimulation occurring at 0.2 M KCl, NH4+ stimulates activity to a lesser extent, extent, whereas Na+ and Li+ have no effect on CofE activity. A mixture of Mn2+, Mg2+, and K+ is the most effective
Mg2+
divalent cation requirement, maximum CofE activity is observed with the addition of 10 mM MnCl2. Reactions containing 10 mM either MgCl2 or CoCl2 produce 40% of the F420-2 of that is produced with MnCl2. The combination of 5 mM MgCl2 and 2-5 mM of MnCl2 supports the highest activity
Mn2+
Na+
highest activity in presence of both Na+ and Mn2+, assay in presence of 100 mM NaCl, 5 mM MnCl2
NH4+
CofE absolutely requires a monovalent cation for activity, the greatest extent of activation is achieved by K+, NH4+ stimulates activity to a lesser extent, whereas Na+ and Li+ have no effect on CofE activity. A mixture of Mn2+, Mg2+, and K+ is the most effective
Mn2+
there are two Mn2+-binding sites per monomer within close proximity of the GDP alpha and beta-phosphate groups
Mn2+
divalent cation requirement, maximum CofE activity is observed with the addition of 10 mM MnCl2. Reactions containing 10 mM either MgCl2 or CoCl2 produce 40% of the F420-2 of that is produced with MnCl2. The combination of 5 mM MgCl2 and 2-5 mM of MnCl2 supports the highest activity
Mn2+
highest activity in presence of both Na+ and Mn2+, assay in presence of 100 mM NaCl, 5 mM MnCl2
