5.1.3.31: D-tagatose 3-epimerase
This is an abbreviated version!
For detailed information about D-tagatose 3-epimerase, go to the full flat file.
Word Map on EC 5.1.3.31
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5.1.3.31
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d-fructose
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d-psicose
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epimerization
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d-allulose
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cichorii
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ketohexose
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tumefaciens
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izumoring
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l-fuculose
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d-arabinose
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d-sorbose
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aldohexose
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bioproduction
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dpease
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l-xylulose
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synthesis
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loti
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mesorhizobium
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epimerizes
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low-calorie
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d-ribulose
- 5.1.3.31
- d-fructose
- d-psicose
-
epimerization
- d-allulose
- cichorii
-
ketohexose
- tumefaciens
-
izumoring
- l-fuculose
- d-arabinose
- d-sorbose
-
aldohexose
-
bioproduction
- dpease
- l-xylulose
- synthesis
- loti
-
mesorhizobium
-
epimerizes
-
low-calorie
- d-ribulose
Reaction
Synonyms
D-TE, DTE, ketose 3-epimerase, L-RE, L-ribulose 3-epimerase, L-TE, MJ1311, MJ1311p, PcDTE, RsDTE, RSP_3671, ycjR
ECTree
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Renatured
Renatured on EC 5.1.3.31 - D-tagatose 3-epimerase
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recombinant enzyme from Escherichia coli inclusion bodies by treatment with 20 ml 10 mM Tris-HCl buffer, pH 7.5, containing 1.0 mM EDTA, 4% Triton X-100, incubation at room temperature for 30 min, twice. Then the inclusion bodies are treated with denaturant solution containing 50 mM Tris-HCl buffer, pH 7.5, containing 6 M guanidine-HCl, 0.2 M NaCl, 1 mM EDTA overnight at 4°C. The solubilized enzyme (100 mg protein in 100 ml solution) is gently dropped into 1 l of refolding buffer containing 0.1 M Tris-HCl, pH 7.5, 1 mM MnCl2, and 0.4 M L-arginine, and incubated for 16 h at 4°C, followed by ultrafiltration