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5.1.3.31: D-tagatose 3-epimerase

This is an abbreviated version!
For detailed information about D-tagatose 3-epimerase, go to the full flat file.

Word Map on EC 5.1.3.31

Reaction

D-psicose
=
D-fructose

Synonyms

D-TE, DTE, ketose 3-epimerase, L-RE, L-ribulose 3-epimerase, L-TE, MJ1311, MJ1311p, PcDTE, RsDTE, RSP_3671, ycjR

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.31 D-tagatose 3-epimerase

Renatured

Renatured on EC 5.1.3.31 - D-tagatose 3-epimerase

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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme from Escherichia coli inclusion bodies by treatment with 20 ml 10 mM Tris-HCl buffer, pH 7.5, containing 1.0 mM EDTA, 4% Triton X-100, incubation at room temperature for 30 min, twice. Then the inclusion bodies are treated with denaturant solution containing 50 mM Tris-HCl buffer, pH 7.5, containing 6 M guanidine-HCl, 0.2 M NaCl, 1 mM EDTA overnight at 4°C. The solubilized enzyme (100 mg protein in 100 ml solution) is gently dropped into 1 l of refolding buffer containing 0.1 M Tris-HCl, pH 7.5, 1 mM MnCl2, and 0.4 M L-arginine, and incubated for 16 h at 4°C, followed by ultrafiltration