4.2.99.B1: DNA 5'-deoxyribose phosphate lyase
This is an abbreviated version!
For detailed information about DNA 5'-deoxyribose phosphate lyase, go to the full flat file.
Reaction
catalysis of the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site in DNA where a DNA-(apurinic or apyrimidinic site) lyase has already cleaved the C-O-P bond 3' to the apurinic or apyrimidinic site
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Synonyms
5' dRP lyase, 5'-deoxyribose phosphate lyase, 5'-deoxyribose-5-phosphate lyase, 5'-deoxyribosephosphate lyase, 5'-dRP lyase, 5'-RP lyase, 8-kDa domain dRP lyase, AtPolIA, AtPolIB, beta-pol, beta-pol dRP lyase, deoxyribo-5'-phosphate lyase, DNA beta-polymerase, DNA polymerase beta, DNA polymerase beta deoxyribose phosphate lyase, DNA polymerase gamma, DNA polymerase lambda, DNA polymerase theta, dRP lyase, dRPase, HSV-1 Pol, HSV-1-DNA polymerase, Ku70, Li polbeta, mammalian endonuclease VIII-like protein, NEIL1, NEIL2, pol beta, Pol gamma, pol iota, Pol lambda, Pol theta, PolB, polymerase iota, Rev1, TcPolbeta, TcPolbetaPAK, Trf4, UL30
ECTree
KM Value
KM Value on EC 4.2.99.B1 - DNA 5'-deoxyribose phosphate lyase
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0.00021 - 0.0022
25-mer-deoxyoligonucleotide 3'-labeled uracil-containing DNA duplex
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0.00045
28-mer deoxyoligonucleotide with a 5' uracil residue
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pretreated with Eschericia coli uracil N-glycosylase, with concentrations 0.1-2.0 microMol, kcat/Km: 0.24 microMol/min
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0.009 - 0.0097
35-bp-deoxyoligonucleotide duplex containing a uracil residue at position 15
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0.0005
DNA with a 5'-incised apurinic/apyrimidinic site at position 21
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dRP lyase activity of beta-pol. To quantify the kinetic parameters for the release of deoxyribose phosphate from a preincused apurinic/apyrimidinic site-containing DNA, deoxyribose phosphate is examined as a function of apurinic/apyrimidinic site concentration. The catalytic efficiency results in a kcat/Km: 0.00015 mM/s. The catalytic efficiency of the 8-kDa amino-terminal domain is 7fold lower than that of the intact 39-kDa beta-pol
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0.0018
mimic of a preincised DNA duplex with a 5'dRP at the site of the nick
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pH 7.5, 37°C
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0.0006 - 0.007
preincised apurinic/apyrimidinic DNA
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additional information
intact apurinic/apyrimidinic site
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0.00021
25-mer-deoxyoligonucleotide 3'-labeled uracil-containing DNA duplex
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NEIL1
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0.0022
25-mer-deoxyoligonucleotide 3'-labeled uracil-containing DNA duplex
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NEIL2
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0.009
35-bp-deoxyoligonucleotide duplex containing a uracil residue at position 15
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full-length 290-kDa Pol theta, pH 7.5, 37°C, kcat/Km: 0.047 microM/min
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0.0097
35-bp-deoxyoligonucleotide duplex containing a uracil residue at position 15
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98-kDa fragment of Pol theta, pH 7.5, 37°C, kcat/Km: 0.072 microM/min
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0.0006
preincised apurinic/apyrimidinic DNA
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pH 7.4, 37°C, in presence of 0.2 mM EDTA
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0.007
preincised apurinic/apyrimidinic DNA
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recombinant protein, pH 7.4, 37°C, in presence of 0.2 mM EDTA
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additional information
intact apurinic/apyrimidinic site
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similar to that of preincised apurinic/apyrimidinic site
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additional information
additional information
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kinetic analysis of dRP lyase activity of the C-terminal domain of Rev1 using a 34-bp duplex DNA substrate. The substrate contained an 18mer DNA strand with 6-FAM at the 3'- end and 5'-RP flap at the margin of a single-nucleotide gap
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