4.2.1.82: xylonate dehydratase
This is an abbreviated version!
For detailed information about xylonate dehydratase, go to the full flat file.
Word Map on EC 4.2.1.82
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4.2.1.82
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caulobacter
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crescentus
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d-xylose
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glycol
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biomass
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1,2,4-butanetriol
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aldolase
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semialdehyde
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pentose
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putida
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2-keto
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ethylene
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dehydration
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azospirillum
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leguminosarum
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halophilic
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dahms
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alpha-ketoglutarate
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haloferax
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lactococcus
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rhizobium
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fe-s
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6-phosphogluconate
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2-ketoacid
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four-step
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pentonate
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trifolii
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volcanii
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brasilense
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lactis
- 4.2.1.82
-
caulobacter
- crescentus
- d-xylose
- glycol
- biomass
- 1,2,4-butanetriol
- aldolase
- semialdehyde
- pentose
- putida
-
2-keto
- ethylene
- dehydration
-
azospirillum
-
leguminosarum
-
halophilic
-
dahms
- alpha-ketoglutarate
-
haloferax
-
lactococcus
-
rhizobium
- fe-s
- 6-phosphogluconate
-
2-ketoacid
-
four-step
-
pentonate
- trifolii
- volcanii
- brasilense
- lactis
Reaction
Synonyms
CcXyDHT, D-xylo-aldonate dehydratase, D-xylonate dehydratase, dehydratase, D-xylonate, XAD, XYLD, xylonate dehydratase, YjhG
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Cloned
Cloned on EC 4.2.1.82 - xylonate dehydratase
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heterologously expressed in Escherichia coli
PCR product cloned into pET19b. The resulting plasmid harvested from Escherichia coli JM109, sequenced, and transformed into Escherichia coli Rosetta(DE3)-pLysS expression strain. In-frame deletion mutant amplified and ligated into pTA131, sequenced, and transformed in Haloferax volcanii H26 DELTApyrE2
xylXABCD, xylXAD, xylXD, xylD or xylX ligated into the vector pJTmcs using the KpnI, XbaI, and XmaJI restriction sites, under the control of the constitutive tac promoter, expressed in Pseudomonas putida S12
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