4.1.1.63: protocatechuate decarboxylase
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For detailed information about protocatechuate decarboxylase, go to the full flat file.
Word Map on EC 4.1.1.63
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4.1.1.63
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muconic
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3-hydroxybenzoic
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3,4,5-trihydroxybenzoic
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enterobacter
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cis,cis-muconate
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synthesis
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vanillate
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dithiothreitol
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cloacae
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lignin-derived
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regioselective
- 4.1.1.63
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muconic
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3-hydroxybenzoic
- 3,4,5-trihydroxybenzoic
- enterobacter
- cis,cis-muconate
- synthesis
- vanillate
- dithiothreitol
- cloacae
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lignin-derived
-
regioselective
Reaction
Synonyms
3,4-Dihydrobenzoate decarboxylase, 3,4-dihydroxybenzoate decarboxylase, AroY, Decarboxylase, protocatechuate, ECL_01944
ECTree
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Application
Application on EC 4.1.1.63 - protocatechuate decarboxylase
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synthesis
engineering of Pseudomonas putida KT2440 to produce muconate from either aromatic molecules or sugars. Coexpression of protocatechuate decarboxylase and associated proteins reduces protocatechuate accumulation and enhances muconate production relative to strains expressing the protocatechuate decarboxylase alone. In bioreactor experiments, coexpression increases the specific productivity of muconate from the lignin monomer p-coumarate by 50% and results in a titer of >15 g/l. In strains engineered to produce muconate from glucose, coexpression more than triples the titer, yield, productivity, and specific productivity, with the best strain producing 4.92±0.48 g/l muconate
synthesis
increase in protocatechuate decarboxylase activity in Escherichia coli through enhanced expression of both protocatechuate decarboxylase AroYand kpdB encoding the B-subunit of 4-hydroxybenzoate decarboxylase. This permits expression of protocatechuate decarboxylase activity at a level approximately 14fold greater than the strain with aroY only. The expression level of AroY increases, apparently as a function of the coexpression of AroY and KpdB
synthesis
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production of the 4-hydroxybenzoate, protocatechuate, and catechol in Escherichia coli. To enhance endogenous biosynthesis of 4-hydroxybenzoate, native chorismate pyruvate lyase ubiC is overexpressed. 4-Hydroxybenzoate is converted to protocatechuate by hydroxylase pobA from Pseudomonas aeruginosa. Catechol is produced by the additional coexpression of protocatechuate decarboxylase from Enterobacter cloacae. Systematic expression of appropriate pathway elements in phenylalanine overproducing Escherichia coli enables initial titers of 32, 110, and 81 mg/l for 4-hydroxybenzoate, protocatechuate, and catechol, respectively. Disruption of chorismate mutase/prephenate dehydratase (pheA) to preserve endogenous chorismate then allows maximum titers of 277, 454, and 451 mg/l, respectively, at glucose yields of 5.8, 9.7, and 14.3% of their respective theoretical maxima
synthesis
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engineering of Pseudomonas putida KT2440 to produce muconate from either aromatic molecules or sugars. Coexpression of protocatechuate decarboxylase and associated proteins reduces protocatechuate accumulation and enhances muconate production relative to strains expressing the protocatechuate decarboxylase alone. In bioreactor experiments, coexpression increases the specific productivity of muconate from the lignin monomer p-coumarate by 50% and results in a titer of >15 g/l. In strains engineered to produce muconate from glucose, coexpression more than triples the titer, yield, productivity, and specific productivity, with the best strain producing 4.92±0.48 g/l muconate
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