3.7.1.13: 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate hydrolase

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For detailed information about 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate hydrolase, go to the full flat file.

Reaction

(2E,4E)-6-(2-aminophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate

Synonyms

CarC, HOPDA hydrolase

ECTree

3 Hydrolases

3.7 Acting on carbon-carbon bonds

3.7.1 In ketonic substances

3.7.1.13 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate hydrolase

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Results	in table
167	AA Sequence
4	Cloned(Commentary)
1	Cofactor
1	Crystallization (Commentary)
2	kcat/KM [mM/s]
4	KM Value [mM]
9	Molecular Weight [Da]
14	Organism
4	Pathway
2	pH Optimum
3	pH Range
2	Protein Variants
4	Purification (Commentary)
1	Reaction
7	Reference
2	Specific Activity [micromol/min/mg]
19	Substrates and Products (Substrate)
7	Subunits
6	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
2	Temperature Stability [°C]
4	Turnover Number [1/s]

Reference

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Reference on EC 3.7.1.13 - 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate hydrolase

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676944

Riddle, R.R.; Gibbs, P.R.; Willson, R.C.; Benedik, M.J.

Purification and properties of 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid hydrolase involved in microbial degradation of carbazole

Protein Expr. Purif.

182-189

2003

Pseudomonas sp., Pseudomonas sp. LD2

12651123

707350

Habe, H.; Morii, K.; Fushinobu, S.; Nam, J.W.; Ayabe, Y.; Yoshida, T.; Wakagi, T.; Yamane, H.; Nojiri, H.; Omori, T.

Crystal structure of a histidine-tagged serine hydrolase involved in the carbazole degradation (CarC enzyme)

Biochem. Biophys. Res. Commun.

303

631-639

2003

Pseudomonas resinovorans, Janthinobacterium sp. J3 (Q84II3), Pseudomonas resinovorans CA10

12659866

707775

Nojiri, H.; Taira, H.; Iwata, K.; Morii, K.; Nam, J.W.; Yoshida, T.; Habe, H.; Nakamura, S.; Shimizu, K.; Yamane, H.; Omori, T.

Purification and characterization of meta-cleavage compound hydrolase from a carbazole degrader Pseudomonas resinovorans strain CA10

Biosci. Biotechnol. Biochem.

36-45

2003

Pseudomonas resinovorans (Q9AQM4), Pseudomonas resinovorans CA10 (Q9AQM4)

12619671