3.5.1.29: 2-(acetamidomethylene)succinate hydrolase

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For detailed information about 2-(acetamidomethylene)succinate hydrolase, go to the full flat file.

Reaction

Synonyms

(E)-2-(acetamidomethylene)succinate hydrolase, AAMS amidohydrolase, AAMSAH, alpha-(N-acetylaminomethylene)succinic acid amidohydrolase, alpha-(N-acetylaminomethylene)succinic acid hydrolase, compound A hydrolase, E-2AMS hydrolase, mlr6787

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.1 In linear amides

                3.5.1.29 2-(acetamidomethylene)succinate hydrolase

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Results	in table
168	AA Sequence
1	CAS Registry Number
4	Cloned(Commentary)
2	Crystallization (Commentary)
5	General Information
1	General Stability
27	Inhibitors
4	KM Value [mM]
1	Metals/Ions
5	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
29	Organism
3	Pathway
6	pH Optimum
1	pH Range
1	pH Stability
5	Protein Variants
6	Purification (Commentary)
1	Reaction
3	Reaction Type
11	Reference
3	Specific Activity [micromol/min/mg]
16	Substrates and Products (Substrate)
8	Subunits
16	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
2	Temperature Stability [°C]
3	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 3.5.1.29 - 2-(acetamidomethylene)succinate hydrolase

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

50

Mesorhizobium loti

-

the enzyme is stable at 50°C for 10 min, the half-lives of the enzyme are 193.6, 77.3, 21.6, and 13.6 min at 50, 55, 58, and 60°C, respectively

699701

70 - 90

Pseudomonas sp.

-

at pH 8 and 70°C stable for 1 min, loses activity completely in 1 min at 90°C

172028