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3.4.24.B34: Vipera ammodytes ammodytes metalloproteinase VaF1

This is an abbreviated version!
For detailed information about Vipera ammodytes ammodytes metalloproteinase VaF1, go to the full flat file.

Reaction

Cleavage of the Lys413-/-Leu414 bond of alpha-chain of human fibrinogen. Cleavage of Ala14-/-Leu15 and Tyr16-/-Leu17 in insulin B chain. Non-hemorrhagic proteinase =

Synonyms

VaF1

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.B34 Vipera ammodytes ammodytes metalloproteinase VaF1

Substrates Products

Substrates Products on EC 3.4.24.B34 - Vipera ammodytes ammodytes metalloproteinase VaF1

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
bovine factor X + H2O
?
show the reaction diagram
slow degradation. The heavy chain offactor X is more susceptible to hydrolysis by the enzyme than the light chain whose hydrolysis is observed only after prolonged incubation
-
-
?
bovine fibronectin + H2O
?
show the reaction diagram
-
-
-
?
bovine plasminogen + H2O
?
show the reaction diagram
slow degradation. Very limited proteolysis, giving rise to multiple products with molecular masses between 40 and 60 kDa
-
-
?
bovine prothrombin + H2O
?
show the reaction diagram
slow degradation. Two major cleavage products, of 60 and 28 kDa, accumulate after extended incubation. N-terminal sequencing shows them to be prethrombin-1 and fragment 1, indicating the cleavage of prothrombin at the Ser157-/-Gly158 bond
-
-
?
factor X + H2O
?
show the reaction diagram
partial hydrolysis
-
-
?
Human fibrinogen + H2O
?
show the reaction diagram
-
-
-
?
human fibrinogen alpha-chain + H2O
?
show the reaction diagram
cleavage of the Lys413-/-Leu414 bond of alpha-chain of fibrinogen. Fibrinogen beta- and gamma-chains remain intact even after 3 h incubation
-
-
?
human type collagen IV + H2O
?
show the reaction diagram
-
-
-
?
insulin B chain + H2O
?
show the reaction diagram
cleavage of two peptide bonds is detected, Ala14-/-Leu15 and Tyr16-/-Leu17
-
-
?
Nidogen + H2O
?
show the reaction diagram
hydrolysis at the Ser322-/-Phe323 bond
-
-
?
plasminogen + H2O
?
show the reaction diagram
partial hydrolysis
-
-
?
prothrombin + H2O
?
show the reaction diagram
partial hydrolysis
-
-
?
additional information
?
-