3.4.11.B6: Thermococcus kodakarensis deblocking aminopeptidase

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For detailed information about Thermococcus kodakarensis deblocking aminopeptidase, go to the full flat file.

Reaction

TkDAP1 prefers to degrade unblocked, polar substrate (L-leucyl 4-nitroanilide) and blocked non-polar peptide (N-acetyl-Ala-Ala-Ala). TkDAP2 has high activity towards both unblocked substrates (L-leucyl 4-nitroanilide and Ala-Ala-Ala), irrespective of polarity. =

Synonyms

deblocking aminopeptidase, TK0775, TK0781, TkDAP1, TkDAP2

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.11 Aminopeptidases

                3.4.11.B6 Thermococcus kodakarensis deblocking aminopeptidase

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Results	in table
2	AA Sequence
1	Cloned(Commentary)
2	Expression
1	Inhibitors
4	Molecular Weight [Da]
2	Organism
2	pH Optimum
2	pH Range
1	Purification (Commentary)
1	Reaction
1	Reference
6	Substrates and Products (Substrate)
4	Subunits
6	Synonyms
2	Temperature Optimum [°C]
2	Temperature Range [°C]

Substrates Products

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Substrates Products on EC 3.4.11.B6 - Thermococcus kodakarensis deblocking aminopeptidase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

Ala-Ala-Ala 4-nitroanilide + H2O

L-alanine + Ala-Ala-Ala 4-nitroanilide

Thermococcus kodakarensis

Q5JH87, Q5JHA5

about 10% of the activity with L-leucyl 4-nitroanilide

719118

-

-

?

Ala-Ala-Ala-4-nitroanilide + H2O

L-alanine + Ala-Ala-Ala 4-nitroanilide

Thermococcus kodakarensis

Q5JH87, Q5JHA5

about 10% of the activity with Leu-4-nitroanilide

719118

-

-

?

L-leucyl 4-nitroanilide + H2O

L-leucine + 4-nitroaniline

Thermococcus kodakarensis

Q5JH87, Q5JHA5

highest specific activity compared to other substrates tested

719118

-

-

?

N-acetyl-Ala-Ala-Ala + H2O

N-acetyl-L-alanine + Ala-Ala

2 entries

N-acetyl-L-leucyl 4-nitroanilide + H2O

N-acetyl-L-leucine + 4-nitroaniline

Thermococcus kodakarensis

Q5JH87, Q5JHA5

about 30% of the activity with L-leucyl 4-nitroanilide

719118

-

-

?

N-acetyl-Ala-Ala-Ala + H2O

N-acetyl-L-alanine + Ala-Ala

Thermococcus kodakarensis

Q5JH87, Q5JHA5

about 65% of the activity with L-leucyl 4-nitroanilide

719118

-

-

?

N-acetyl-Ala-Ala-Ala + H2O

N-acetyl-L-alanine + Ala-Ala

Thermococcus kodakarensis

Q5JH87, Q5JHA5

about 65% of the activity with Leu-4-nitroanilide

719118

-

-

?