3.3.2.12: oxepin-CoA hydrolase
This is an abbreviated version!
For detailed information about oxepin-CoA hydrolase, go to the full flat file.
Reaction
Synonyms
EC 3.7.1.16, ECH-Aa, MaoC, oxepin-CoA hydrolase/3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase (NADP+), paaZ, PaaZ-ECH
ECTree
3.3.2.12 oxepin-CoA hydrolaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.3.2.12 - oxepin-CoA hydrolase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
crotonyl-CoA + H2O
(R)-3-hydroxybutyryl-CoA
-
the enzyme shows 1% activity with crotonyl-CoA compared to oxepin-CoA
-
-
?
2-oxepin-2(3H)-ylideneacetyl-CoA + H2O
3-oxo-5,6-dehydrosuberyl-CoA semialdehyde
-
-
-
?
2-oxepin-2(3H)-ylideneacetyl-CoA + H2O
3-oxo-5,6-dehydrosuberyl-CoA semialdehyde
-
addition of purified PaaZ enzyme to enzymatically produced epoxide and oxepin in the presence of PaaG protein leads to a complete NADP+-dependent conversion of epoxide and oxepin into 3-oxo-5,6-dehydrosuberyl-CoA. PaaZ functions as an oxepin-CoA hydrolase/3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase catalyzing the two-step conversion of the oxepin-CoA via the open-chain aldehyde intermediate to 3-oxo-5,6-dehydrosuberyl-CoA
-
-
?
oxepin-CoA + H2O
3-oxo-5,6-dehydrosuberyl-CoA semialdehyde
-
100% activity
main product
-
?
additional information
?
-
-
the bifunctional protein also use 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde and NADP+ as substrate yielding 3-oxo-5,6-dehydrosuberyl-CoA as main product
-
-
?
additional information
?
-
-
the bifunctional protein also use 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde and NADP+ as substrate yielding 3-oxo-5,6-dehydrosuberyl-CoA as main product. The PaaZ-ECH domain acts as (R)-specific hydratase and shows no activity with (S)-3-hydroxybutyryl-CoA
-
-
?
additional information
?
-
-
bifunctional protein, catalyzing the reactions of 2-oxepin-2(3H)-ylideneacetyl-CoA hydrolase, EC 3.3.2.12, and 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase, EC 1.17.1.7
-
-
?
additional information
?
-
bifunctional protein, catalyzing the reactions of 2-oxepin-2(3H)-ylideneacetyl-CoA hydrolase, EC 3.3.2.12, and 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase, EC 1.17.1.7
-
-
?
additional information
?
-
-
enzyme additionally shows enoyl-CoA hydratase activity involved in supplying (R)-3-hydroxyacyl-CoA from the beta-oxidation pathway to polyhydroxyalkanoate biosynthetic pathway in the fadB mutant Escherichia coli strain
-
-
?
