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3.2.1.93: alpha,alpha-phosphotrehalase

This is an abbreviated version!
For detailed information about alpha,alpha-phosphotrehalase, go to the full flat file.

Word Map on EC 3.2.1.93

Reaction

alpha,alpha-trehalose 6-phosphate
+
H2O
=
D-glucose
 +
D-glucose 6-phosphate

Synonyms

Alpha,alpha-phosphotrehalase, BlTreA, phosphotrehalase, TreA, trehalose-6-phosphate hydrolase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.93 alpha,alpha-phosphotrehalase

Engineering

Engineering on EC 3.2.1.93 - alpha,alpha-phosphotrehalase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D203E
no detectable activity with alpha,alpha-trehalose 6-phosphate or 4-nitrophenyl alpha-D-glucopyranoside
D329E
no detectable activity with alpha,alpha-trehalose 6-phosphate or 4-nitrophenyl alpha-D-glucopyranoside
E254D
no detectable activity with alpha,alpha-trehalose 6-phosphate or 4-nitrophenyl alpha-D-glucopyranoside
H281A
kcat/Km for 4-nitrophenyl alpha-D-glucopyranoside is reduced 5.3fold as compared to wild-type value, kcat/Km for alpha,alpha-trehalose 6-phosphate is reduced 5.25fold as compared to wild-type value
H282A
kcat/Km for 4-nitrophenyl alpha-D-glucopyranoside is reduced 2.65fold as compared to wild-type value, kcat/Km for alpha,alpha-trehalose 6-phosphate is reduced 2.6fold as compared to wild-type value
K284A
kcat/Km for 4-nitrophenyl alpha-D-glucopyranoside is reduced 1.5fold as compared to wild-type value, kcat/Km for alpha,alpha-trehalose 6-phosphate is reduced 1.5fold as compared to wild-type value
K292A
kcat/Km for 4-nitrophenyl alpha-D-glucopyranoside is reduced 265fold as compared to wild-type value, kcat/Km for alpha,alpha-trehalose 6-phosphate is reduced 189fold as compared to wild-type value
D203E
-
no detectable activity with alpha,alpha-trehalose 6-phosphate or 4-nitrophenyl alpha-D-glucopyranoside
-
E254D
-
no detectable activity with alpha,alpha-trehalose 6-phosphate or 4-nitrophenyl alpha-D-glucopyranoside
-
H281A
-
kcat/Km for 4-nitrophenyl alpha-D-glucopyranoside is reduced 5.3fold as compared to wild-type value, kcat/Km for alpha,alpha-trehalose 6-phosphate is reduced 5.25fold as compared to wild-type value
-
K284A
-
kcat/Km for 4-nitrophenyl alpha-D-glucopyranoside is reduced 1.5fold as compared to wild-type value, kcat/Km for alpha,alpha-trehalose 6-phosphate is reduced 1.5fold as compared to wild-type value
-
K292A
-
kcat/Km for 4-nitrophenyl alpha-D-glucopyranoside is reduced 265fold as compared to wild-type value, kcat/Km for alpha,alpha-trehalose 6-phosphate is reduced 189fold as compared to wild-type value
-
additional information