3.2.1.161: beta-apiosyl-beta-glucosidase

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For detailed information about beta-apiosyl-beta-glucosidase, go to the full flat file.

Reaction

Synonyms

Dnbglu2, FH, furcatin hydrolase, isoflavonoid 7-O-apiosylglucosidase, isoflavonoid 7-O-beta-apiosyl-glucoside beta-glucosidase, isoflavonoid-7-O-beta[D-apiofuranosyl-(1->6)-beta-D-glucoside] disaccharidase

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.161 beta-apiosyl-beta-glucosidase

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Results	in table
7	AA Sequence
2	CAS Registry Number
3	Cloned(Commentary)
8	Inhibitors
2	kcat/KM [mM/s]
29	KM Value [mM]
2	Localization
1	Metals/Ions
6	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
6	Organism
2	Pathway
4	pH Optimum
2	pH Range
1	Posttranslational Modification
8	Protein Variants
4	Purification (Commentary)
1	Reaction
3	Reaction Type
6	Reference
6	Source Tissue
3	Specific Activity [micromol/min/mg]
40	Substrates and Products (Substrate)
3	Subunits
10	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Stability [°C]
13	Turnover Number [1/s]

Engineering

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Engineering on EC 3.2.1.161 - beta-apiosyl-beta-glucosidase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

A419V

Viburnum furcatum

-

furcatin: 40%, pNP beta-primeveroside: 78% and kcat/Km (min-1 * mM-1) = 1.3, pAP beta-glucoside: 56%, pNP beta-glucoside: 127% activity compared to wild-type

698652

A419W

Viburnum furcatum

-

furcatin: 26%, pNP beta-primeveroside: 24% and kcat/Km (min-1 * mM-1) = 0.59, pAP beta-glucoside: 43%, pNP beta-glucoside: 39% activity compared to wild-type

698652

E501A

Viburnum furcatum

-

furcatin: 0.1% activity compared to wild-type

698652

E501D

Viburnum furcatum

-

furcatin: 0.2% activity compared to wild-type

698652

E501L

Viburnum furcatum

-

furcatin: 0.1% activity compared to wild-type

698652

E501Q

Viburnum furcatum

-

furcatin: 0.1% activity compared to wild-type

698652

S504A

Viburnum furcatum

-

furcatin: 113%, pNP beta-primeveroside: 62% and kcat/Km (min-1 * mM-1) = 0.75, pAP beta-glucoside: 87%, pNP beta-glucoside: 87% activity compared to wild-type

698652

S504W

Viburnum furcatum

-

furcatin: 1.4%, pNP beta-primeveroside: 1.3% and kcat/Km (min-1 * mM-1) = 0.013, pAP beta-glucoside: 20%, pNP beta-glucoside: 28% activity compared to wild-type

698652