2.7.2.7: butyrate kinase
This is an abbreviated version!
For detailed information about butyrate kinase, go to the full flat file.
Word Map on EC 2.7.2.7
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2.7.2.7
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clostridium
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acetobutylicum
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phosphotransbutyrylase
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butanol
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butyrate-producing
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butyryl-coa
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butyryl
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coa-transferase
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solventogenesis
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acidogenic
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phosphotransacetylase
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butyryl-coa:acetate
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askha
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solventogenic
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roseburia
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acetone-butanol-ethanol
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faecalibacterium
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nutrition
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biotechnology
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synthesis
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industry
- 2.7.2.7
- clostridium
- acetobutylicum
- phosphotransbutyrylase
- butanol
-
butyrate-producing
- butyryl-coa
-
butyryl
-
coa-transferase
-
solventogenesis
-
acidogenic
- phosphotransacetylase
-
butyryl-coa:acetate
-
askha
-
solventogenic
- roseburia
-
acetone-butanol-ethanol
-
faecalibacterium
- nutrition
- biotechnology
- synthesis
- industry
Reaction
Synonyms
BK, BKI, BKII, Buk, buk1, Buk2, butyrate kinase, butyrate kinase 2, butyrate kinase I, butyrate kinase II, butyrokinase, kinase (phosphorylating), butyrate, kinase, butyrate (phosphorylating)
ECTree
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Engineering
Engineering on EC 2.7.2.7 - butyrate kinase
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E332D
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the mutant shows 3.82% of wild type butyrate kinase II activity
E332L
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the mutant shows 0.34% of wild type butyrate kinase II activity
E332Q
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the mutant shows 3.02% of wild type butyrate kinase II activity
E334D
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the mutant shows 0.29% of wild type butyrate kinase II activity
E334L
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the mutant shows0.58% of wild type butyrate kinase II activity
E334Q
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the mutant shows 0.41% of wild type butyrate kinase II activity
H183L
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the mutant shows 0.06% of wild type butyrate kinase II activity
H183Q
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the mutant shows 0.19% of wild type butyrate kinase II activity
R150K
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the mutant shows 21% of wild type butyrate kinase II activity
R215F E332D
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the mutant shows 0.38% of wild type butyrate kinase II activity
R215K
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the mutant shows 0.44% of wild type butyrate kinase II activity
additional information
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metabolic engineering studies of Clostridium acetobutylicum strain M5 to produce butanol without acetone
additional information
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semi-automated reverse engineering algorithm. The reconstructed metabolic network was used to create a genome-scale model that correctly characterized the butyrate kinase knock-out and the asolventogenic M5 pSOL1 megaplasmid degenerate strains. Systematic gene knock-out simulations performed to identify a set of genes encoding clostridial enzymes essential for growth in silico.
additional information
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metabolic engineering studies of Clostridium acetobutylicum strain M5 to produce butanol without acetone
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additional information
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pta-deleted mutant, BK activity increases by 44%, ack-deleted mutant, similar activity as wild-type