2.4.2.7: adenine phosphoribosyltransferase

This is an abbreviated version!
For detailed information about adenine phosphoribosyltransferase, go to the full flat file.

Reaction

Synonyms

adenine phosphoribosyl transferase, adenine phosphoribosylpyrophosphate transferase, adenine phosphoribosyltransferase, adenine phosphoribosyltransferase 2, adenione PRTase, adenosine phosphoribosyltransferase, adenylate pyrophosphorylase, adenylic pyrophosphorylase, AMP pyrophosphorylase, AMP-pyrophosphate phosphoribosyltransferase, AMP:pyrophosphate phospho-D-ribosyltransferase, APRT, APRT1, APRT2, APRTase, APRTh, apT, APT1, APT2, APT4, APT5, ApTR, FtAPRT, gpT-1, hAPRT, More, phosphoribosyltransferase, adenine, ScAPRT, SmAPRT1, Smp_054360, SSO2342, transphosphoribosidase, TtAPRT2, TTC1249, TTC1250, TTH1250, TTHA1614, TthAPRT, TthHB27APRT, TT_C1249, TT_C1250, type I PRT

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.2 Pentosyltransferases

                2.4.2.7 adenine phosphoribosyltransferase

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Results	in table
5	Activating Compound
33808	AA Sequence
3	Application
1	CAS Registry Number
27	Cloned(Commentary)
15	Crystallization (Commentary)
1379	Disease/ Diagnostics
9	Expression
67	General Information
11	General Stability
1	IC50 Value
121	Inhibitors
10	kcat/KM [mM/s]
36	Ki Value [mM]
94	KM Value [mM]
9	Localization
77	Metals/Ions
36	Molecular Weight [Da]
46	Natural Substrates/ Products (Substrates)
257	Organism
9	Pathway
79	PDB ID
41	pH Optimum
7	pH Range
3	pI Value
45	Protein Variants
40	Purification (Commentary)
12	Reaction
1	Reaction Type
92	Reference
55	Source Tissue
36	Specific Activity [micromol/min/mg]
13	Storage Stability
202	Substrates and Products (Substrate)
46	Subunits
146	Synonyms
1	Systematic Name
34	Temperature Optimum [°C]
1	Temperature Range [°C]
28	Temperature Stability [°C]
30	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 2.4.2.7 - adenine phosphoribosyltransferase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-15°C, stable for months

Escherichia coli

-

638155

-20--80°C, purified recombinant His-tagged enzyme, 70-80% activity reamining after 70 days

Thermus thermophilus

Q5SHW6

758716

-20°C, purified enzyme, 2-3 weeks, considerable loss of activity

Escherichia coli

-

638147

-20°C, purified enzyme, 6 mM 5-phospho-alpha-D-ribose 1-diphosphate, 2 mM MgCl2, stable for at least a month

Schizosaccharomyces pombe

-

638161

-70°C, 50 mM Tris-HCl, pH 7.4, 5 mM MgCl2, 10 mM KCl, 10% glycerol, stable

Brassica juncea

-

638159

-70°C, purified enzyme, 5 mM MgCl2, 0.1 mM 5-phospho-alpha-D-ribose 1-diphosphate, stable for upt to 2 months

Homo sapiens

-

638151

-70°C, purified enzyme, at least 6 months

Leishmania donovani

-

638004

-79°C, purified enzyme, 0.1 mg/ml protein, 0.1 M potassium phosphate buffer, pH 7, stable for several months

Escherichia coli

-

638147

-80°C, stable for at least 3 weeks

Catharanthus roseus

-

638152

2-4°C, purified enzyme in the ampholine gradient solution, half-life of 2-4 weeks, dilution inactivates

Rattus norvegicus

-

638149, 638156

4°C, purified recombinant His-tagged enzyme, 85% activity remaining after 70 days

Thermus thermophilus

Q5SHW6

758716

unstable at 4°C or at -20°C

Catharanthus roseus

-

638152