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2.4.1.140: alternansucrase

This is an abbreviated version!
For detailed information about alternansucrase, go to the full flat file.

Word Map on EC 2.4.1.140

Reaction

[Glc(alpha1-6)Glcalpha(1-3)]n
+ 2 sucrose =
[Glc(alpha1-6)Glcalpha(1-3)]n+2
 + 2 beta-D-fructofuranose

Synonyms

alternansucrase, ASR, glucansucrase, glucosyltransferase, sucrose-1,6(3)-alpha-glucan 6(3)-alpha-, Lcalt, sucrose:1,6-, 1,3-alpha-D-glucan 3-alpha- and 6-alpha-D-glucosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.140 alternansucrase

Cloned

Cloned on EC 2.4.1.140 - alternansucrase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
expression in Solanum tuberosum. The Asr transcript levels correlated well with alternan accumulation in tuber juices
-
gene asr, recombinant expression of wild-type enzyme and truncated mutants in Escherichia coli strain BL21(DE3)
gene Lcalt, rcombinant expression in Escherichia coli strain BL21(DE3)
recombinant expression of the large alternansucrase (2057 amino acids) is hindered in Escherichia coli due to poor enzyme solubility and protein degradation. A truncated variant deleted of the APY repeats but harboring four C-terminal CW-like repeats display a high specific activity and the same specificity of product synthesis as the native enzyme. It is more soluble and suffers less degradation than full length alternansucrase