1.21.3.3: reticuline oxidase

This is an abbreviated version!
For detailed information about reticuline oxidase, go to the full flat file.

Reaction

Synonyms

(S)-reticuline oxidase, BBE, BBE-like 13, BBE-like 15, BBE-like 28, BBE1, BBL, berberine bridge enzyme, berberine bridge enzyme-like, berberine bridge enzyme-like 28, berberine bridge-forming enzyme, berberine-bridge-forming enzyme, EC 1.5.3.9, flavin-dependent oxidase, monolignol oxidoreductase, reticuline oxidase, tetrahydroprotoberberine synthase

ECTree

     1 Oxidoreductases

         1.21 Catalysing the reaction X-H + Y-H = X-Y

             1.21.3 With oxygen as acceptor

                1.21.3.3 reticuline oxidase

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Results	in table
1	Activating Compound
48	AA Sequence
3	Application
1	CAS Registry Number
16	Cloned(Commentary)
12	Cofactor
6	Crystallization (Commentary)
1	Expression
2	General Information
13	Inhibitors
2	kcat/KM [mM/s]
7	KM Value [mM]
6	Localization
4	Molecular Weight [Da]
24	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
36	Organism
11	Pathway
13	PDB ID
5	pH Optimum
3	pH Range
2	Posttranslational Modification
14	Protein Variants
6	Purification (Commentary)
1	Reaction
7	Reaction Type
35	Reference
20	Source Tissue
4	Storage Stability
70	Substrates and Products (Substrate)
2	Subunits
40	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Range [°C]
14	Turnover Number [1/s]

Application

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Application on EC 1.21.3.3 - reticuline oxidase

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

medicine

Papaver somniferum

P93479

a Saccharomyces cerevisiae strain is engineered to express seven heterologous enzymes (Papaper somniferum norcoclaurine 6-O-methyltransferase (Ps6OMT), Papaver somniferum 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase 2 (Ps4'OMT), Papapver somniferum coclaurine N-methyltransferase (PsCNMT), Papaver somniferum berberine bridge enzyme (PsBBE), Thalictrum flavum scoulerine 9-O-methyltransferase (TfS9OMT), Thalictrum flavum canadine synthase (TfCAS), and Arabidopsis thaliana cytochrome P450 reductase 1 (CPR)), resulting in protoberberine alkaloid production from a simple benzylisoquinoline alkaloid precursor. A number of strategies are implemented to improve flux through the pathway, including enzyme variant screening, genetic copy number variation, and culture optimization. This leads to an over 70-fold increase in canadine titer up to 1.8 mg/l. Increased canadine titers enable extension of the pathway to produce berberine, a major constituent of several traditional medicines in a microbial host. This strain is viable at pilot scale

743234

synthesis

2 entries

synthesis

Eschscholzia californica

P30986

substrate tuning by introducing a fluoro moiety at one potential reactive carbon center switches the reaction to the formation of exclusively one regioisomer with perfect enantioselectivity. The formation of 11-hydroxy-functionalized tetrahydroprotoberberines instead of the commonly formed 9-hydroxy-functionalized products from 1,2,3,4-tetrahydroisoquinolines can be successfully promoted

727325

synthesis

Papaver somniferum

P93479

heterologous production of berberine and the optimization of the engineered biosynthetic pathway from rac-norlaudanosoline to (S)-canadine in yeast involving the recombinant berberine reductase

743234