1.2.3.14: abscisic-aldehyde oxidase
This is an abbreviated version!
For detailed information about abscisic-aldehyde oxidase, go to the full flat file.
Word Map on EC 1.2.3.14
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1.2.3.14
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1.2.3.1
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9-cis-epoxycarotenoids
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indole-3-aldehyde
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moco
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wilty
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psaos
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1.2.1.37
- 1.2.3.14
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1.2.3.1
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9-cis-epoxycarotenoids
- indole-3-aldehyde
- moco
- wilty
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psaos
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1.2.1.37
Reaction
Synonyms
AAO, AAO1, AAO2, AAO3, ABA aldehyde oxidase, ABA-specific aldehyde oxidase, abscisic aldehyde oxidase, abscisic aldehyde oxidase 3, AO-3, AO3, AOd, AOdelta, Arabidopsis aldehyde oxidase 3, EC 1.2.3.1
ECTree
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Substrates Products
Substrates Products on EC 1.2.3.14 - abscisic-aldehyde oxidase
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REACTION DIAGRAM
1-naphthaldehyde + H2O + O2
naphthalene-1-carboxylate + H2O2
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substrate activity assay
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?
abscisic aldehyde + 2,6-dichloroindophenol
abscisic acid + H2O2
2,6-dichloroindophenol i.e. DCIP used as electron acceptor, natural electron acceptor is oxygen
rate of H2O2 formation increases in the presence of superoxide dismutase, indicating that in addition to the two-electron reduction of molecular oxygen, AAO1 and AAO3 also catalyze a one-electron transfer to molecular oxygen, leading to the formation of superoxide ion, O2-
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?
benzaldehyde + 2,6-dichloroindophenol
benzoic acid + H2O2
2,6-dichloroindophenol i.e. DCIP used as electron acceptor, natural electron acceptor is oxygen
rate of H2O2 formation increases in the presence of superoxide dismutase, indicating that in addition to the two-electron reduction of molecular oxygen, AAO1 and AAO3 also catalyze a one-electron transfer to molecular oxygen, leading to the formation of superoxide ion, O2-
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?
heptaldehyde + 2,6-dichloroindophenol
heptanoic acid + H2O2
2,6-dichloroindophenol i.e. DCIP used as electron acceptor, natural electron acceptor is oxygen
rate of H2O2 formation increases in the presence of superoxide dismutase, indicating that in addition to the two-electron reduction of molecular oxygen, AAO1 and AAO3 also catalyze a one-electron transfer to molecular oxygen, leading to the formation of superoxide ion, O2-
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?
indole-3-carbaldehyde + 2,6-dichloroindophenol
indole-3-carboxylate + H2O2
2,6-dichloroindophenol i.e. DCIP used as electron acceptor, natural electron acceptor is oxygen
rate of H2O2 formation increases in the presence of superoxide dismutase, indicating that in addition to the two-electron reduction of molecular oxygen, AAO1 and AAO3 also catalyze a one-electron transfer to molecular oxygen, leading to the formation of superoxide ion, O2-
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?
NADH + O2
NAD+ + O2-
oxidation of NADH by AAO1 and AAO3, no oxidation of NADPH by AAO1 or AAO3
for confirmation, O2--dependent reduction of cytochrome c monitored, oxidation of NADH by AAO1 and AAO3 does not result in detectable levels of H2O2
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additional information
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confirmation of superoxide generation by AAO1 and AAO3 by monitoring the reduction of the tetrazolium salt XTT due to O2-
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abscisate + H2O2
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the AAO3 gene product plays a major role in abscisic acid biosynthesis in seed
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abscisic aldehyde + H2O + O2
abscisate + H2O2
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the enzyme catalyzes the final step of abscisic acid biosynthesis, specifically in rosette leaves
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abscisic aldehyde + H2O + O2
abscisate + H2O2
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the enzyme catalyzes the final step of abscisic acid biosynthesis. AAO3 is the AAO that plays a major role in abscisic acid biosynthesis in seeds as well as in leaves
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abscisic aldehyde + H2O + O2
abscisate + H2O2
the enzyme catalyzes the final step of abscisic acid biosynthesis. NO detectable activity in guard cells of nonstressed rosette or wet-control leaves
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indole-3-carboxylate + H2O2
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substrate activity assay
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?