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1.14.19.28: sn-1 stearoyl-lipid 9-desaturase

This is an abbreviated version!
For detailed information about sn-1 stearoyl-lipid 9-desaturase, go to the full flat file.

Word Map on EC 1.14.19.28

Reaction

a 1-stearoyl-2-acyl-[glycerolipid]
+ 2 reduced ferredoxin [iron-sulfur] cluster +
O2
+ 2 H+ =
a 1-oleoyl-2-acyl-[glycerolipid]
+ 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O

Synonyms

9des, acyl-lipid DELTA9-desaturase, ADS1, ADS2, BH720_24295, DELTA9 acyl-lipid desaturase, DELTA9 acyl–lipid desaturase, DELTA9 desaturase, DELTA9-desaturase, DELTA9-fatty acid desaturase, DELTA9-fatty acid desaturase-like protein, desC, desC acyl–lipid desaturase, desC1, DesC1 DELTA9, sn-1, FAD9, More, PA3FAD9, PA8FAD9, TcDELTA9DES

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.28 sn-1 stearoyl-lipid 9-desaturase

Engineering

Engineering on EC 1.14.19.28 - sn-1 stearoyl-lipid 9-desaturase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Thermostichus vulcanus
establishment of transgenic lines of Nicotiana tabacum expressing hybrid genes of Synechocystis sp. PCC 6803 DELTA12 (desA) acyl-lipid desaturase and Synechococcus vulcanus DELTA9 (desC) acyl-lipid desaturase with or without sequence coding for transit peptide of Rubisco small subunit of Arabidopsis thaliana under control of a constitutive promoter. Reliable increase of linoleic acid portion (C18:2, DELTA9,12) accompanied with decrease of alpha-linolenic acid (C18:3, DELTA9,12,15) relative amount is detected for plants expressing hybrid desA::licBM3 gene. No reliable changes are detected in fatty acid profiles and unsaturation index of plants transformed with DELTA9 desaturase gene desC::licBM3 lacking signals of intracellular targeting while expression of this gene with Arabidopsis thaliana Rubisco small subunit transit peptide sequence caused growth of C18:3 alpha-linolenic acid part simultaneously with reduction of C18:2 linoleic acid part, as well as increase of unsaturation index. No changes in relative amount of DELTA9monounsaturated fatty acids were observed in any of studied lines. All plants expressing desaturase genes exhibited enhanced levels of superoxide dismutase (SOD) activity after cold treatment in contrast to control lines with suppressed SOD activity after cold treatment