1.14.13.59: L-lysine N6-monooxygenase (NADPH)
This is an abbreviated version!
For detailed information about L-lysine N6-monooxygenase (NADPH), go to the full flat file.
Word Map on EC 1.14.13.59
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1.14.13.59
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fad
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siderophore
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flavoproteins
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ornithine
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nicotinamide
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c4a-hydroperoxyflavin
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hydroxamate
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nadp+
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hydroxamate-containing
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oxygen-dependent
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5-aminovalerate
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aerobactin
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glutarate
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semialdehyde
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flavin-containing
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iodoacetate
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fumigatus
- 1.14.13.59
- fad
-
siderophore
- flavoproteins
- ornithine
- nicotinamide
-
c4a-hydroperoxyflavin
- hydroxamate
- nadp+
-
hydroxamate-containing
-
oxygen-dependent
- 5-aminovalerate
- aerobactin
- glutarate
- semialdehyde
-
flavin-containing
- iodoacetate
- fumigatus
Reaction
Synonyms
EC 1.13.12.10, EC 13.12.10, flavin-dependent lysine monooxygenase, flavin-dependent N6-lysine monooxygenase, IucD, LH, lysine monooxygenase, Lysine N(6)-hydroxylase, Lysine N6-hydroxylase, lysine N6-monooxygenase, lysine: N6-hydroxylase, lysine:N(6)-hydroxylase, Lysine:N6-hydroxylase, MbsG, N-hydroxylating monooxygenase, NbtG, Oxygenase, lysine N6-mono-
ECTree
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Purification
Purification on EC 1.14.13.59 - L-lysine N6-monooxygenase (NADPH)
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recombinant enzyme form IucD439, in which the sequence encoding the IucD protein is fused in frame to the amino-terminal sequence of beta-galactosidase
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recombinant His-tagged wild-type and P14G mutant enzymes from strain M15-2 to homogeneity by gel filtration, ion exchange and nickel affinity chromatography
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recombinant His8-MBP-tagged wild-type and mutant K184A enzymes, as well as seleno-L-methionine-labeled enzyme from Escherichia coli TOP10 cells by nickel affinity chromatography, dialysis, tag cleavage by TEV protease, followed by a second step of nickel affinity chromatography and by MBP-tracking affinity chromatography, eluting the detagged enzyme, followed by dialysis
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