1.14.11.B15: S-(L-glutamyl)-[peptidyl-carrier protein]-3-hydroxylase (3-hydroxylating, erythro-hydroxy-L-glutamyl-[peptidyl-carrier-protein]-forming)
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Reaction
Synonyms
KtzP
ECTree
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KCat KM Value
KCat KM Value on EC 1.14.11.B15 - S-(L-glutamyl)-[peptidyl-carrier protein]-3-hydroxylase (3-hydroxylating, erythro-hydroxy-L-glutamyl-[peptidyl-carrier-protein]-forming)
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additional information
additional information
nonhydrolyzable coenzyme A analogs are developed and used to determine the kinetic parameters for KtzP-catalyzed hydroxylation of glutamic acid bound to the carrier protein. To determine the kinetic parameters of KtzP catalyzed hydroxylation, the problem of the labile thioester bond is circumvented by using synthetic coenzyme A analogs coupled to glutamic acid where the thioester is replaced by a hydrolytically stable amide bond
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