Sequence of RHOA_HUMAN
EC Number:3.6.5.2
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
Reaction
GTP + H2O = GDP + phosphate
Other sequences found for EC No. 3.6.5.2
General information:
Sequence
0 MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYVADIEVDG KQVELALWDT
60 AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT PEVKHFCPNV PIILVGNKKD
120 LRNDEHTRRE LAKMKQEPVK PEEGRDMANR IGAFGYMECS AKTKDGVREV FEMATRAALQ
180 ARRGKKKSGC LVL
Download this sequence
in fasta formatDownload all sequences for 3.6.5.2
in fasta format
in csv (Excel, OpenOffice) formatSequence related references
Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
469468
Yeramian P.,Chardin P.,Madaule P.,Tavitian A.
Nucleotide sequence of human rho cDNA clone 12.
Nucleic Acids Res.
15
1869-1869
1987
469469
Fagan K.P.,Oliveira L.,Pittler S.J.
Sequence of rho small GTP-binding protein cDNAs from human retina and identification of novel 5' end cloning artifacts.
Exp. Eye Res.
59
235-237
1994
469473
Bechtel S.,Rosenfelder H.,Duda A.,Schmidt C.P.,Ernst U.,Wellenreuther R.,Mehrle A.,Schuster C.,Bahr A.,Bloecker H.,Heubner D.,Hoerlein A.,Michel G.,Wedler H.,Koehrer K.,Ottenwaelder B.,Poustka A.,Wiemann S.,Schupp I.
The full-ORF clone resource of the German cDNA consortium.
BMC Genomics
8
399-399
2007
469474
Muzny D.M.,Scherer S.E.,Kaul R.,Wang J.,Yu J.,Sudbrak R.,Buhay C.J.,Chen R.,Cree A.,Ding Y.,Dugan-Rocha S.,Gill R.,Gunaratne P.,Harris R.A.,Hawes A.C.,Hernandez J.,Hodgson A.V.,Hume J.,Jackson A.,Khan Z.M.,Kovar-Smith C.,Lewis L.R.,Lozado R.J.,Metzker M.L.,Milosavljevic A.,Miner G.R.,Morgan M.B.,Nazareth L.V.,Scott G.,Sodergren E.,Song X.-Z.,Steffen D.,Wei S.,Wheeler D.A.,Wright M.W.,Worley K.C.,Yuan Y.,Zhang Z.,Adams C.Q.,Ansari-Lari M.A.,Ayele M.,Brown M.J.,Chen G.,Chen Z.,Clendenning J.,Clerc-Blankenburg K.P.,Chen R.,Chen Z.,Davis C.,Delgado O.,Dinh H.H.,Dong W.,Draper H.,Ernst S.,Fu G.,Gonzalez-Garay M.L.,Garcia D.K.,Gillett W.,Gu J.,Hao B.,Haugen E.,Havlak P.,He X.,Hennig S.,Hu S.,Huang W.,Jackson L.R.,Jacob L.S.,Kelly S.H.,Kube M.,Levy R.,Li Z.,Liu B.,Liu J.,Liu W.,Lu J.,Maheshwari M.,Nguyen B.-V.,Okwuonu G.O.,Palmeiri A.,Pasternak S.,Perez L.M.,Phelps K.A.,Plopper F.J.,Qiang B.,Raymond C.,Rodriguez R.,Saenphimmachak C.,Santibanez J.,Shen H.,Shen Y.,Subramanian S.,Tabor P.E.,Verduzco D.,Waldron L.,Wang J.,Wang J.,Wang Q.,Williams G.A.,Wong G.K.-S.,Yao Z.,Zhang J.,Zhang X.,Zhao G.,Zhou J.,Zhou Y.,Nelson D.,Lehrach H.,Reinhardt R.,Naylor S.L.,Yang H.,Olson M.,Weinstock G.,Gibbs R.A.
The DNA sequence, annotation and analysis of human chromosome 3.
Nature
440
1194-1198
2006
469475
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
Genome Res.
14
2121-2127
2004
469476
Moscow J.A.,He R.,Gudas J.M.,Cowan K.H.
Utilization of multiple polyadenylation signals in the human RHOA protooncogene.
Gene
144
229-236
1994
469477
Nemoto Y.,Namba T.,Teru-uchi T.,Ushikubi F.,Morii N.,Narumiya S.
A rho gene product in human blood platelets. I. Identification of the platelet substrate for botulinum C3 ADP-ribosyltransferase as rhoA protein.
J. Biol. Chem.
267
20916-20920
1992
469478
Just I.,Selzer J.,Wilm M.,von Eichel-Streiber C.,Mann M.,Aktories K.
Glucosylation of Rho proteins by Clostridium difficile toxin B.
Nature
375
500-503
1995
469479
Moscow J.A.,Morrow C.S.,He R.,Mullenbach G.T.,Cowan K.H.
Structure and function of the 5'-flanking sequence of the human cytosolic selenium-dependent glutathione peroxidase gene (hgpx1).
J. Biol. Chem.
267
5949-5958
1992
469480
Just I.,Wilm M.,Selzer J.,Rex G.,von Eichel-Streiber C.,Mann M.,Aktories K.
The enterotoxin from Clostridium difficile (ToxA) monoglucosylates the Rho proteins.
J. Biol. Chem.
270
13932-13936
1995
469481
Ishizaki T.,Maekawa M.,Fujisawa K.,Okawa K.,Iwamatsu A.,Fujita A.,Watanabe N.,Saito Y.,Kakizuka A.,Morii N.,Narumiya S.
The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase.
EMBO J.
15
1885-1893
1996
469482
Matsui T.,Amano M.,Yamamoto T.,Chihara K.,Nakafuku M.,Ito M.,Nakano T.,Okawa K.,Iwamatsu A.,Kaibuchi K.
Rho-associated kinase, a novel serine/threonine kinase, as a putative target for small GTP binding protein Rho.
EMBO J.
15
2208-2216
1996
469483
Quilliam L.A.,Lambert Q.T.,Mickelson-Young L.A.,Westwick J.K.,Sparks A.B.,Kay B.K.,Jenkins N.A.,Gilbert D.J.,Copeland N.G.,Der C.J.
Isolation of a NCK-associated kinase, PRK2, an SH3-binding protein and potential effector of Rho protein signaling.
J. Biol. Chem.
271
28772-28776
1996
469484
Selzer J.,Hofmann F.,Rex G.,Wilm M.,Mann M.,Just I.,Aktories K.
Clostridium novyi alpha-toxin-catalyzed incorporation of GlcNAc into Rho subfamily proteins.
J. Biol. Chem.
271
25173-25177
1996
469485
Vincent S.,Settleman J.
The PRK2 kinase is a potential effector target of both Rho and Rac GTPases and regulates actin cytoskeletal organization.
Mol. Cell. Biol.
17
2247-2256
1997
469486
Cachero T.G.,Morielli A.D.,Peralta E.G.
The small GTP-binding protein RhoA regulates a delayed rectifier potassium channel.
Cell
93
1077-1085
1998
469487
Ren Y.,Li R.,Zheng Y.,Busch H.
Cloning and characterization of GEF-H1, a microtubule-associated guanine nucleotide exchange factor for Rac and Rho GTPases.
J. Biol. Chem.
273
34954-34960
1998
469488
Houssa B.,de Widt J.,Kranenburg O.,Moolenaar W.H.,van Blitterswijk W.J.
Diacylglycerol kinase theta binds to and is negatively regulated by active RhoA.
J. Biol. Chem.
274
6820-6822
1999
469489
Pastey M.K.,Crowe J.E. Jr.,Graham B.S.
RhoA interacts with the fusion glycoprotein of respiratory syncytial virus and facilitates virus-induced syncytium formation.
J. Virol.
73
7262-7270
1999
469490
Reynaud C.,Fabre S.,Jalinot P.
The PDZ protein TIP-1 interacts with the Rho effector rhotekin and is involved in Rho signaling to the serum response element.
J. Biol. Chem.
275
33962-33968
2000
469491
Sawada N.,Itoh H.,Yamashita J.,Doi K.,Inoue M.,Masatsugu K.,Fukunaga Y.,Sakaguchi S.,Sone M.,Yamahara K.,Yurugi T.,Nakao K.
cGMP-dependent protein kinase phosphorylates and inactivates RhoA.
Biochem. Biophys. Res. Commun.
280
798-805
2001
469492
Klussmann E.,Edemir B.,Pepperle B.,Tamma G.,Henn V.,Klauschenz E.,Hundsrucker C.,Maric K.,Rosenthal W.
Ht31: the first protein kinase A anchoring protein to integrate protein kinase A and Rho signaling.
FEBS Lett.
507
264-268
2001
469493
Arthur W.T.,Ellerbroek S.M.,Der C.J.,Burridge K.,Wennerberg K.
XPLN, a guanine nucleotide exchange factor for RhoA and RhoB, but not RhoC.
J. Biol. Chem.
277
42964-42972
2002
469494
Shao F.,Merritt P.M.,Bao Z.,Innes R.W.,Dixon J.E.
A Yersinia effector and a Pseudomonas avirulence protein define a family of cysteine proteases functioning in bacterial pathogenesis.
Cell
109
575-588
2002
469495
Lanning C.C.,Ruiz-Velasco R.,Williams C.L.
Novel mechanism of the co-regulation of nuclear transport of SmgGDS and Rac1.
J. Biol. Chem.
278
12495-12506
2003
469496
Wing M.R.,Snyder J.T.,Sondek J.,Harden T.K.
Direct activation of phospholipase C-epsilon by Rho.
J. Biol. Chem.
278
41253-41258
2003
469497
Shao F.,Vacratsis P.O.,Bao Z.,Bowers K.E.,Fierke C.A.,Dixon J.E.
Biochemical characterization of the Yersinia YopT protease: cleavage site and recognition elements in Rho GTPases.
Proc. Natl. Acad. Sci. U.S.A.
100
904-909
2003
469498
Yuce O.,Piekny A.,Glotzer M.
An ECT2-centralspindlin complex regulates the localization and function of RhoA.
J. Cell Biol.
170
571-582
2005
469499
Kamijo K.,Ohara N.,Abe M.,Uchimura T.,Hosoya H.,Lee J.S.,Miki T.
Dissecting the role of Rho-mediated signaling in contractile ring formation.
Mol. Biol. Cell
17
43-55
2006
469500
Wolf A.,Keil R.,Gotzl O.,Mun A.,Schwarze K.,Lederer M.,Huttelmaier S.,Hatzfeld M.
The armadillo protein p0071 regulates Rho signalling during cytokinesis.
Nat. Cell Biol.
8
1432-1440
2006
469501
Bristow J.M.,Sellers M.H.,Majumdar D.,Anderson B.,Hu L.,Webb D.J.
The Rho-family GEF Asef2 activates Rac to modulate adhesion and actin dynamics and thereby regulate cell migration.
J. Cell Sci.
122
4535-4546
2009
469502
Stirling L.,Williams M.R.,Morielli A.D.
Dual roles for RHOA/RHO-kinase in the regulated trafficking of a voltage-sensitive potassium channel.
Mol. Biol. Cell
20
2991-3002
2009
469503
Worby C.A.,Mattoo S.,Kruger R.P.,Corbeil L.B.,Koller A.,Mendez J.C.,Zekarias B.,Lazar C.,Dixon J.E.
The fic domain: regulation of cell signaling by adenylylation.
Mol. Cell
34
93-103
2009
469504
Chen Y.,Yang Z.,Meng M.,Zhao Y.,Dong N.,Yan H.,Liu L.,Ding M.,Peng H.B.,Shao F.
Cullin mediates degradation of RhoA through evolutionarily conserved BTB adaptors to control actin cytoskeleton structure and cell movement.
Mol. Cell
35
841-855
2009
469505
Yarbrough M.L.,Li Y.,Kinch L.N.,Grishin N.V.,Ball H.L.,Orth K.
AMPylation of Rho GTPases by Vibrio VopS disrupts effector binding and downstream signaling.
Science
323
269-272
2009
469506
Chatterjee A.,Wang L.,Armstrong D.L.,Rossie S.
Activated Rac1 GTPase translocates protein phosphatase 5 to the cell membrane and stimulates phosphatase activity in vitro.
J. Biol. Chem.
285
3872-3882
2010
469507
Berg T.J.,Gastonguay A.J.,Lorimer E.L.,Kuhnmuench J.R.,Li R.,Fields A.P.,Williams C.L.
Splice variants of SmgGDS control small GTPase prenylation and membrane localization.
J. Biol. Chem.
285
35255-35266
2010
469508
Boulter E.,Garcia-Mata R.,Guilluy C.,Dubash A.,Rossi G.,Brennwald P.J.,Burridge K.
Regulation of Rho GTPase crosstalk, degradation and activity by RhoGDI1.
Nat. Cell Biol.
12
477-483
2010
469509
Zaoui K.,Benseddik K.,Daou P.,Salaun D.,Badache A.
ErbB2 receptor controls microtubule capture by recruiting ACF7 to the plasma membrane of migrating cells.
Proc. Natl. Acad. Sci. U.S.A.
107
18517-18522
2010
469510
Burkard T.R.,Planyavsky M.,Kaupe I.,Breitwieser F.P.,Buerckstuemmer T.,Bennett K.L.,Superti-Furga G.,Colinge J.
Initial characterization of the human central proteome.
BMC Syst. Biol.
5
17-17
2011
469511
Naji L.,Pacholsky D.,Aspenstrom P.
ARHGAP30 is a Wrch-1-interacting protein involved in actin dynamics and cell adhesion.
Biochem. Biophys. Res. Commun.
409
96-102
2011
469512
Cao X.X.,Xu J.D.,Xu J.W.,Liu X.L.,Cheng Y.Y.,Li Q.Q.,Xu Z.D.,Liu X.P.
RACK1 promotes breast carcinoma migration/metastasis via activation of the RhoA/Rho kinase pathway.
Breast Cancer Res. Treat.
126
555-563
2011
469513
Wallace S.W.,Magalhaes A.,Hall A.
The Rho target PRK2 regulates apical junction formation in human bronchial epithelial cells.
Mol. Cell. Biol.
31
81-91
2011
469514
Dubash A.D.,Koetsier J.L.,Amargo E.V.,Najor N.A.,Harmon R.M.,Green K.J.
The GEF Bcr activates RhoA/MAL signaling to promote keratinocyte differentiation via desmoglein-1.
J. Cell Biol.
202
653-666
2013
469515
Li D.,Dammer E.B.,Lucki N.C.,Sewer M.B.
cAMP-stimulated phosphorylation of diaphanous 1 regulates protein stability and interaction with binding partners in adrenocortical cells.
Mol. Biol. Cell
24
848-857
2013
469516
Jank T.,Bogdanovic X.,Wirth C.,Haaf E.,Spoerner M.,Boehmer K.E.,Steinemann M.,Orth J.H.,Kalbitzer H.R.,Warscheid B.,Hunte C.,Aktories K.
A bacterial toxin catalyzing tyrosine glycosylation of Rho and deamidation of Gq and Gi proteins.
Nat. Struct. Mol. Biol.
20
1273-1280
2013
469517
Genth H.,Pauillac S.,Schelle I.,Bouvet P.,Bouchier C.,Varela-Chavez C.,Just I.,Popoff M.R.
Haemorrhagic toxin and lethal toxin from Clostridium sordellii strain vpi9048: molecular characterization and comparative analysis of substrate specificity of the large clostridial glucosylating toxins.
Cell. Microbiol.
16
1706-1721
2014
469518
Gao K.,Tang W.,Li Y.,Zhang P.,Wang D.,Yu L.,Wang C.,Wu D.
Front-signal-dependent accumulation of the RHOA inhibitor FAM65B at leading edges polarizes neutrophils.
J. Cell Sci.
128
992-1000
2015
469519
Vaca Jacome A.S.,Rabilloud T.,Schaeffer-Reiss C.,Rompais M.,Ayoub D.,Lane L.,Bairoch A.,Van Dorsselaer A.,Carapito C.
N-terminome analysis of the human mitochondrial proteome.
Proteomics
15
2519-2524
2015
469520
Mardakheh F.K.,Self A.,Marshall C.J.
RHO binding to FAM65A regulates Golgi reorientation during cell migration.
J. Cell Sci.
129
4466-4479
2016
469521
Shimizu H.,Toma-Fukai S.,Saijo S.,Shimizu N.,Kontani K.,Katada T.,Shimizu T.
Structure-based analysis of the guanine nucleotide exchange factor SmgGDS reveals armadillo-repeat motifs and key regions for activity and GTPase binding.
J. Biol. Chem.
292
13441-13448
2017
469522
Chen M.,Pan H.,Sun L.,Shi P.,Zhang Y.,Li L.,Huang Y.,Chen J.,Jiang P.,Fang X.,Wu C.,Chen Z.
Structure and regulation of human epithelial cell transforming 2 protein.
Proc. Natl. Acad. Sci. U.S.A.
117
1027-1035
2020
469523
Vabres P.,Sorlin A.,Kholmanskikh S.S.,Demeer B.,St-Onge J.,Duffourd Y.,Kuentz P.,Courcet J.B.,Carmignac V.,Garret P.,Bessis D.,Boute O.,Bron A.,Captier G.,Carmi E.,Devauchelle B.,Genevieve D.,Gondry-Jouet C.,Guibaud L.,Lafon A.,Mathieu-Dramard M.,Thevenon J.,Dobyns W.B.,Bernard G.,Polubothu S.,Faravelli F.,Kinsler V.A.,Thauvin C.,Faivre L.,Ross M.E.,Riviere J.B.
Postzygotic inactivating mutations of RHOA cause a mosaic neuroectodermal syndrome.
Nat. Genet.
51
1438-1441
2019
469524
Liu W.,Zhou Y.,Peng T.,Zhou P.,Ding X.,Li Z.,Zhong H.,Xu Y.,Chen S.,Hang H.C.,Shao F.
Nepsilon-fatty acylation of multiple membrane-associated proteins by Shigella IcsB effector to modulate host function.
Nat. Microbiol.
3
996-1009
2018
469525
Wei Y.,Zhang Y.,Derewenda U.,Liu X.,Minor W.,Nakamoto R.K.,Somlyo A.V.,Somlyo A.P.,Derewenda Z.S.
Crystal structure of RhoA-GDP and its functional implications.
Nat. Struct. Biol.
4
699-703
1997
469526
Ihara K.,Muraguchi S.,Kato M.,Shimizu T.,Shirakawa M.,Kuroda S.,Kaibuchi K.,Hakoshima T.
Crystal structure of human RhoA in a dominantly active form complexed with a GTP analogue.
J. Biol. Chem.
273
9656-9666
1998
469527
Maesaki R.,Shimizu T.,Ihara K.,Kuroda S.,Kaibuchi K.,Hakoshima T.
Biochemical and crystallographic characterization of a Rho effector domain of the protein serine/threonine kinase N in a complex with RhoA.
J. Struct. Biol.
126
166-170
1999
469528
Shimizu T.,Ihara K.,Maesaki R.,Kuroda S.,Kaibuchi K.,Hakoshima T.
An open conformation of switch I revealed by the crystal structure of a Mg2+-free form of RHOA complexed with GDP. Implications for the GDP/GTP exchange mechanism.
J. Biol. Chem.
275
18311-18317
2000
469529
Graham D.L.,Lowe P.N.,Grime G.W.,Marsh M.,Rittinger K.,Smerdon S.J.,Gamblin S.J.,Eccleston J.F.
MgF(3)(-) as a transition state analog of phosphoryl transfer.
Chem. Biol.
9
375-381
2002
469530
Snyder J.T.,Worthylake D.K.,Rossman K.L.,Betts L.,Pruitt W.M.,Siderovski D.P.,Der C.J.,Sondek J.
Structural basis for the selective activation of Rho GTPases by Dbl exchange factors.
Nat. Struct. Biol.
9
468-475
2002
469531
Longenecker K.,Read P.,Lin S.-K.,Somlyo A.P.,Nakamoto R.K.,Derewenda Z.S.
Structure of a constitutively activated RhoA mutant (Q63L) at 1.55 A resolution.
Acta Crystallogr. D
59
876-880
2003
469532
Shimizu H.,Toma-Fukai S.,Kontani K.,Katada T.,Shimizu T.
GEF mechanism revealed by the structure of SmgGDS-558 and farnesylated RhoA complex and its implication for a chaperone mechanism.
Proc. Natl. Acad. Sci. U.S.A.
115
9563-9568
2018
