Sequence of EST1_HUMAN
EC Number:3.1.1.56
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
Reaction
4-methylumbelliferyl acetate + H2O = 4-methylumbelliferone + acetate
General information:
Sequence
0 MWLRAFILAT LSASAAWGHP SSPPVVDTVH GKVLGKFVSL EGFAQPVAIF LGIPFAKPPL
60 GPLRFTPPQP AEPWSFVKNA TSYPPMCTQD PKAGQLLSEL FTNRKENIPL KLSEDCLYLN
120 IYTPADLTKK NRLPVMVWIH GGGLMVGAAS TYDGLALAAH ENVVVVTIQY RLGIWGFFST
180 GDEHSRGNWG HLDQVAALRW VQDNIASFGG NPGSVTIFGE SAGGESVSVL VLSPLAKNLF
240 HRAISESGVA LTSVLVKKGD VKPLAEQIAI TAGCKTTTSA VMVHCLRQKT EEELLETTLK
300 MKFLSLDLQG DPRESQPLLG TVIDGMLLLK TPEELQAERN FHTVPYMVGI NKQEFGWLIP
360 MQLMSYPLSE GQLDQKTAMS LLWKSYPLVC IAKELIPEAT EKYLGGTDDT VKKKDLFLDL
420 IADVMFGVPS VIVARNHRDA GAPTYMYEFQ YRPSFSSDMK PKTVIGDHGD ELFSVFGAPF
480 LKEGASEEEI RLSKMVMKFW ANFARNGNPN GEGLPHWPEY NQKEGYLQIG ANTQAAQKLK
540 DKEVAFWTNL FAKKAVEKPP QTEHIEL
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Sequence related references
Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
464854
Munger J.S.,Shi G.P.,Mark E.A.,Chin D.T.,Gerard C.,Chapman H.A.
A serine esterase released by human alveolar macrophages is closely related to liver microsomal carboxylesterases.
J. Biol. Chem.
266
18832-18838
1991
464855
Kroetz D.L.,McBride O.W.,Gonzalez F.J.
Glycosylation-dependent activity of baculovirus-expressed human liver carboxylesterases: cDNA cloning and characterization of two highly similar enzyme forms.
Biochemistry
32
11606-11617
1993
464856
Shibata F.,Takagi Y.,Kitajima M.,Kuroda T.,Omura T.
Molecular cloning and characterization of a human carboxylesterase gene.
Genomics
17
76-82
1993
464857
Becker A.,Bottcher A.,Lackner K.J.,Fehringer P.,Notka F.,Aslanidis C.,Schmitz G.
Purification, cloning, and expression of a human enzyme with acyl coenzyme A: cholesterol acyltransferase activity, which is identical to liver carboxylesterase.
Arterioscler. Thromb.
14
1346-1355
1994
464858
Islam M.R.,Waheed A.,Shah G.N.,Tomatsu S.,Sly W.S.
Human egasyn binds beta-glucuronidase but neither the esterase active site of egasyn nor the C-terminus of beta-glucuronidase is involved in their interaction.
Arch. Biochem. Biophys.
372
53-61
1999
464859
Ghosh S.
Cholesteryl ester hydrolase in human monocyte/macrophage: cloning, sequencing, and expression of full-length cDNA.
Physiol. Genomics
2
1-8
2000
464860
Alam M.,Ho S.,Vance D.E.,Lehner R.
Heterologous expression, purification, and characterization of human triacylglycerol hydrolase.
Protein Expr. Purif.
24
33-42
2002
464862
Ota T.,Suzuki Y.,Nishikawa T.,Otsuki T.,Sugiyama T.,Irie R.,Wakamatsu A.,Hayashi K.,Sato H.,Nagai K.,Kimura K.,Makita H.,Sekine M.,Obayashi M.,Nishi T.,Shibahara T.,Tanaka T.,Ishii S.,Yamamoto J.,Saito K.,Kawai Y.,Isono Y.,Nakamura Y.,Nagahari K.,Murakami K.,Yasuda T.,Iwayanagi T.,Wagatsuma M.,Shiratori A.,Sudo H.,Hosoiri T.,Kaku Y.,Kodaira H.,Kondo H.,Sugawara M.,Takahashi M.,Kanda K.,Yokoi T.,Furuya T.,Kikkawa E.,Omura Y.,Abe K.,Kamihara K.,Katsuta N.,Sato K.,Tanikawa M.,Yamazaki M.,Ninomiya K.,Ishibashi T.,Yamashita H.,Murakawa K.,Fujimori K.,Tanai H.,Kimata M.,Watanabe M.,Hiraoka S.,Chiba Y.,Ishida S.,Ono Y.,Takiguchi S.,Watanabe S.,Yosida M.,Hotuta T.,Kusano J.,Kanehori K.,Takahashi-Fujii A.,Hara H.,Tanase T.-O.,Nomura Y.,Togiya S.,Komai F.,Hara R.,Takeuchi K.,Arita M.,Imose N.,Musashino K.,Yuuki H.,Oshima A.,Sasaki N.,Aotsuka S.,Yoshikawa Y.,Matsunawa H.,Ichihara T.,Shiohata N.,Sano S.,Moriya S.,Momiyama H.,Satoh N.,Takami S.,Terashima Y.,Suzuki O.,Nakagawa S.,Senoh A.,Mizoguchi H.,Goto Y.,Shimizu F.,Wakebe H.,Hishigaki H.,Watanabe T.,Sugiyama A.,Takemoto M.,Kawakami B.,Yamazaki M.,Watanabe K.,Kumagai A.,Itakura S.,Fukuzumi Y.,Fujimori Y.,Komiyama M.,Tashiro H.,Tanigami A.,Fujiwara T.,Ono T.,Yamada K.,Fujii Y.,Ozaki K.,Hirao M.,Ohmori Y.,Kawabata A.,Hikiji T.,Kobatake N.,Inagaki H.,Ikema Y.,Okamoto S.,Okitani R.,Kawakami T.,Noguchi S.,Itoh T.,Shigeta K.,Senba T.,Matsumura K.,Nakajima Y.,Mizuno T.,Morinaga M.,Sasaki M.,Togashi T.,Oyama M.,Hata H.,Watanabe M.,Komatsu T.,Mizushima-Sugano J.,Satoh T.,Shirai Y.,Takahashi Y.,Nakagawa K.,Okumura K.,Nagase T.,Nomura N.,Kikuchi H.,Masuho Y.,Yamashita R.,Nakai K.,Yada T.,Nakamura Y.,Ohara O.,Isogai T.,Sugano S.
Complete sequencing and characterization of 21,243 full-length human cDNAs.
Nat. Genet.
36
40-45
2004
464863
Martin J.,Han C.,Gordon L.A.,Terry A.,Prabhakar S.,She X.,Xie G.,Hellsten U.,Chan Y.M.,Altherr M.,Couronne O.,Aerts A.,Bajorek E.,Black S.,Blumer H.,Branscomb E.,Brown N.C.,Bruno W.J.,Buckingham J.M.,Callen D.F.,Campbell C.S.,Campbell M.L.,Campbell E.W.,Caoile C.,Challacombe J.F.,Chasteen L.A.,Chertkov O.,Chi H.C.,Christensen M.,Clark L.M.,Cohn J.D.,Denys M.,Detter J.C.,Dickson M.,Dimitrijevic-Bussod M.,Escobar J.,Fawcett J.J.,Flowers D.,Fotopulos D.,Glavina T.,Gomez M.,Gonzales E.,Goodstein D.,Goodwin L.A.,Grady D.L.,Grigoriev I.,Groza M.,Hammon N.,Hawkins T.,Haydu L.,Hildebrand C.E.,Huang W.,Israni S.,Jett J.,Jewett P.B.,Kadner K.,Kimball H.,Kobayashi A.,Krawczyk M.-C.,Leyba T.,Longmire J.L.,Lopez F.,Lou Y.,Lowry S.,Ludeman T.,Manohar C.F.,Mark G.A.,McMurray K.L.,Meincke L.J.,Morgan J.,Moyzis R.K.,Mundt M.O.,Munk A.C.,Nandkeshwar R.D.,Pitluck S.,Pollard M.,Predki P.,Parson-Quintana B.,Ramirez L.,Rash S.,Retterer J.,Ricke D.O.,Robinson D.L.,Rodriguez A.,Salamov A.,Saunders E.H.,Scott D.,Shough T.,Stallings R.L.,Stalvey M.,Sutherland R.D.,Tapia R.,Tesmer J.G.,Thayer N.,Thompson L.S.,Tice H.,Torney D.C.,Tran-Gyamfi M.,Tsai M.,Ulanovsky L.E.,Ustaszewska A.,Vo N.,White P.S.,Williams A.L.,Wills P.L.,Wu J.-R.,Wu K.,Yang J.,DeJong P.,Bruce D.,Doggett N.A.,Deaven L.,Schmutz J.,Grimwood J.,Richardson P.,Rokhsar D.S.,Eichler E.E.,Gilna P.,Lucas S.M.,Myers R.M.,Rubin E.M.,Pennacchio L.A.
The sequence and analysis of duplication-rich human chromosome 16.
Nature
432
988-994
2004
464864
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
Genome Res.
14
2121-2127
2004
464865
Mori M.,Hosokawa M.,Ogasawara Y.,Tsukada E.,Chiba K.
cDNA cloning, characterization and stable expression of novel human brain carboxylesterase.
FEBS Lett.
458
17-22
1999
464866
Brzezinski M.R.,Abraham T.L.,Stone C.L.,Dean R.A.,Bosron W.F.
Purification and characterization of a human liver cocaine carboxylesterase that catalyzes the production of benzoylecgonine and the formation of cocaethylene from alcohol and cocaine.
Biochem. Pharmacol.
48
1747-1755
1994
464867
Schindler R.,Mentlein R.,Feldheim W.
Purification and characterization of retinyl ester hydrolase as a member of the non-specific carboxylesterase supergene family.
Eur. J. Biochem.
251
863-873
1998
464868
Satoh T.,Hosokawa M.
Molecular aspects of carboxylesterase isoforms in comparison with other esterases.
Toxicol. Lett.
82
439-445
1995
464869
Alam M.,Vance D.E.,Lehner R.
Structure-function analysis of human triacylglycerol hydrolase by site-directed mutagenesis: identification of the catalytic triad and a glycosylation site.
Biochemistry
41
6679-6687
2002
464870
Long R.M.,Calabrese M.R.,Martin B.M.,Pohl L.R.
Cloning and sequencing of a human liver carboxylesterase isoenzyme.
Life Sci.
48
PL43-PL49
1991
464871
Zschunke F.,Salmassi A.,Kreipe H.,Buck F.,Parwaresch M.R.,Radzun H.J.
cDNA cloning and characterization of human monocyte/macrophage serine esterase-1.
Blood
78
506-512
1991
464872
Riddles P.W.,Richards L.J.,Bowles M.R.,Pond S.M.
Cloning and analysis of a cDNA encoding a human liver carboxylesterase.
Gene
108
289-292
1991
464873
Pindel E.V.,Kedishvili N.Y.,Abraham T.L.,Brzezinski M.R.,Zhang J.,Dean R.A.,Bosron W.F.
Purification and cloning of a broad substrate specificity human liver carboxylesterase that catalyzes the hydrolysis of cocaine and heroin.
J. Biol. Chem.
272
14769-14775
1997
464874
Zhao B.,Fisher B.J.,St Clair R.W.,Rudel L.L.,Ghosh S.
Redistribution of macrophage cholesteryl ester hydrolase from cytoplasm to lipid droplets upon lipid loading.
J. Lipid Res.
46
2114-2121
2005
464875
Zhao B.,Song J.,St Clair R.W.,Ghosh S.
Stable overexpression of human macrophage cholesteryl ester hydrolase results in enhanced free cholesterol efflux from human THP1 macrophages.
Am. J. Physiol.
292
C405-C412
2007
464876
Crow J.A.,Middleton B.L.,Borazjani A.,Hatfield M.J.,Potter P.M.,Ross M.K.
Inhibition of carboxylesterase 1 is associated with cholesteryl ester retention in human THP-1 monocyte/macrophages.
Biochim. Biophys. Acta
1781
643-654
2008
464877
Zhao B.,Song J.,Ghosh S.
Hepatic overexpression of cholesteryl ester hydrolase enhances cholesterol elimination and in vivo reverse cholesterol transport.
J. Lipid Res.
49
2212-2217
2008
464878
Zhu H.-J.,Patrick K.S.,Yuan H.-J.,Wang J.-S.,Donovan J.L.,DeVane C.L.,Malcolm R.,Johnson J.A.,Youngblood G.L.,Sweet D.H.,Langaee T.Y.,Markowitz J.S.
Two CES1 gene mutations lead to dysfunctional carboxylesterase 1 activity in man: clinical significance and molecular basis.
Am. J. Hum. Genet.
82
1241-1248
2008
464879
Xie S.,Borazjani A.,Hatfield M.J.,Edwards C.C.,Potter P.M.,Ross M.K.
Inactivation of lipid glyceryl ester metabolism in human THP1 monocytes/macrophages by activated organophosphorus insecticides: role of carboxylesterases 1 and 2.
Chem. Res. Toxicol.
23
1890-1904
2010
464880
Bian Y.,Song C.,Cheng K.,Dong M.,Wang F.,Huang J.,Sun D.,Wang L.,Ye M.,Zou H.
An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.
J. Proteomics
96
253-262
2014
464881
Vaca Jacome A.S.,Rabilloud T.,Schaeffer-Reiss C.,Rompais M.,Ayoub D.,Lane L.,Bairoch A.,Van Dorsselaer A.,Carapito C.
N-terminome analysis of the human mitochondrial proteome.
Proteomics
15
2519-2524
2015
464882
Bencharit S.,Morton C.L.,Hyatt J.L.,Kuhn P.,Danks M.K.,Potter P.M.,Redinbo M.R.
Crystal structure of human carboxylesterase 1 complexed with the Alzheimer's drug tacrine. From binding promiscuity to selective inhibition.
Chem. Biol.
10
341-349
2003
464883
Bencharit S.,Morton C.L.,Xue Y.,Potter P.M.,Redinbo M.R.
Structural basis of heroin and cocaine metabolism by a promiscuous human drug-processing enzyme.
Nat. Struct. Biol.
10
349-356
2003
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