Sequence of HLNO_PAENI
EC Number:1.5.99.4
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
Reaction
(S)-nicotine + acceptor + H2O = (S)-6-hydroxynicotine + reduced acceptor
General information:
Sequence
0 MYDAIVVGGG FSGLKAARDL TNAGKKVLLL EGGERLGGRA YSRESRNVPG LRVEIGGAYL
60 HRKHHPRLAA ELDRYGIPTA AASEFTSFRH RLGPTAVDQA FPIPGSEAVA VEAATYTLLR
120 DAHRIDLEKG LENQDLEDLD IPLNEYVDKL DLPPVSRQFL LAWAWNMLGQ PADQASALWM
180 LQLVAAHHYS ILGVVLSLDE VFSNGSADLV DAMSQEIPEI RLQTVVTGID QSGDVVNVTV
240 KDGHAFQAHS VIVATPMNTW RRIVFTPALP ERRRSVIEEG HGGQGLKILI HVRGAEAGIE
300 CVGDGIFPTL YDYCEVSESE RLLVAFTDSG SFDPTDIGAV KDAVLYYLPE VEVLGIDYHD
360 WIADPLFEGP WVAPRVGQFS RVHKELGEPA GRIHFVGSDV SLEFPGYIEG ALETAECAVN
420 AILHS
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
1128738
Schenk S.,Hoelz A.,Kraus B.,Decker K.
Gene structures and properties of enzymes of the plasmid-encoded nicotine catabolism of Arthrobacter nicotinovorans.
J. Mol. Biol.
284
1323-1339
1998
1128739
Schenk S.,Decker K.
Horizontal gene transfer involved in the convergent evolution of the plasmid-encoded enantioselective 6-hydroxynicotine oxidases.
J. Mol. Evol.
48
178-186
1999
1128740
Baitsch D.,Sandu C.,Brandsch R.,Igloi G.L.
Gene cluster on pAO1 of Arthrobacter nicotinovorans involved in degradation of the plant alkaloid nicotine: cloning, purification, and characterization of 2,6-dihydroxypyridine 3-hydroxylase.
J. Bacteriol.
183
5262-5267
2001
1128741
Igloi G.L.,Brandsch R.
Sequence of the 165-kilobase catabolic plasmid pAO1 from Arthrobacter nicotinovorans and identification of a pAO1-dependent nicotine uptake system.
J. Bacteriol.
185
1976-1986
2003
1128742
Decker K.,Bleeg H.
Induction and purification of stereospecific nicotine oxidizing enzymes from Arthrobacter oxidans.
Biochim. Biophys. Acta
105
313-324
1965
1128743
Decker K.,Dai V.D.
Mechanism and specificity of L- and D-6-hydroxynicotine oxidase.
Eur. J. Biochem.
3
132-138
1967
1128744
Dai V.D.,Decker K.,Sund H.
Purification and properties of L-6-hydroxynicotine oxidase.
Eur. J. Biochem.
4
95-102
1968
1128745
Swafford J.R.,Reeves H.C.,Brandsch R.
Localization of the enantiozymes of 6-hydroxy-nicotine oxidase in Arthrobacter oxidans by electron immunochemistry.
J. Bacteriol.
163
792-795
1985
1128746
Fitzpatrick P.F.,Chadegani F.,Zhang S.,Roberts K.M.,Hinck C.S.
Mechanism of the flavoprotein L-hydroxynicotine oxidase: kinetic mechanism, substrate specificity, reaction product, and roles of active-site residues.
Biochemistry
55
697-703
2016
1128747
Fitzpatrick P.F.,Chadegani F.,Zhang S.,Dougherty V.
Mechanism of flavoprotein L-6-hydroxynicotine oxidase: pH and solvent isotope effects and identification of key active site residues.
Biochemistry
56
869-875
2017
1128748
Kachalova G.S.,Bourenkov G.P.,Mengesdorf T.,Schenk S.,Maun H.R.,Burghammer M.,Riekel C.,Decker K.,Bartunik H.D.
Crystal structure analysis of free and substrate-bound 6-hydroxy-L-nicotine oxidase from Arthrobacter nicotinovorans.
J. Mol. Biol.
396
785-799
2010
1128749
Kachalova G.,Decker K.,Holt A.,Bartunik H.D.
Crystallographic snapshots of the complete reaction cycle of nicotine degradation by an amine oxidase of the monoamine oxidase (MAO) family.
Proc. Natl. Acad. Sci. U.S.A.
108
4800-4805
2011
