Sequence of ERYK_SACEN
EC Number:1.14.15.33
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
pikromycin synthase
P48635
Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
397
43725
Reaction
10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = methymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
General information:
Sequence
0 MTTIDEVPGM ADETALLDWL GTMREKQPVW QDRYGVWHVF RHADVQTVLR DTATFSSDPT
60 RVIEGASPTP GMIHEIDPPE HRALRKVVSS AFTPRTISDL EPRIRDVTRS LLADAGESFD
120 LVDVLAFPLP VTIVAELLGL PPMDHEQFGD WSGALVDIQM DDPTDPALAE RIADVLNPLT
180 AYLKARCAER RADPGDDLIS RLVLAEVDGR ALDDEEAANF STALLLAGHI TTTVLLGNIV
240 RTLDEHPAHW DAAAEDPGRI PAIVEEVLRY RPPFPQMQRT TTKATEVAGV PIPADVMVNT
300 WVLSANRDSD AHDDPDRFDP SRKSGGAAQL SFGHGVHFCL GAPLARLENR VALEEIIARF
360 GRLTVDRDDE RLRHFEQIVL GTRHLPVLAG SSPRQSA
Download this sequence
in fasta formatDownload all sequences for 1.14.15.33
in fasta format
in csv (Excel, OpenOffice) formatSequence related references
Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
565364
Stassi D.,Donadio S.,Staver M.J.,Katz L.
Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis.
J. Bacteriol.
175
182-189
1993
565365
Pereda A.,Summers R.,Katz L.
Nucleotide sequence of the ermE distal flank of the erythromycin biosynthesis cluster in Saccharopolyspora erythraea.
Gene
193
65-71
1997
565366
Oliynyk M.,Samborskyy M.,Lester J.B.,Mironenko T.,Scott N.,Dickens S.,Haydock S.F.,Leadlay P.F.
Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338.
Nat. Biotechnol.
25
447-453
2007
565367
Lambalot R.H.,Cane D.E.,Aparicio J.J.,Katz L.
Overproduction and characterization of the erythromycin C-12 hydroxylase, EryK.
Biochemistry
34
1858-1866
1995
565368
Savino C.,Sciara G.,Miele A.E.,Kendrew S.G.,Vallone B.
Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of C-12 hydroxylase EryK from Saccharopolyspora erythraea.
Protein Pept. Lett.
15
1138-1141
2008
565369
Savino C.,Montemiglio L.C.,Sciara G.,Miele A.E.,Kendrew S.G.,Jemth P.,Gianni S.,Vallone B.
Investigating the structural plasticity of a cytochrome P450: three-dimensional structures of P450 EryK and binding to its physiological substrate.
J. Biol. Chem.
284
29170-29179
2009
565370
Montemiglio L.C.,Gianni S.,Vallone B.,Savino C.
Azole drugs trap cytochrome P450 EryK in alternative conformational states.
Biochemistry
49
9199-9206
2010
