Sequence of O54666_AMYMD
EC Number:6.2.1.74
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
3-amino-5-hydroxybenzoate-[acyl-carrier protein] ligase
O54666
Amycolatopsis mediterranei
4735
495219
Reaction
ATP + 3-amino-5-hydroxybenzoate + a holo-[acyl-carrier protein] = 3-amino-5-hydroxybenzoyl-[acyl-carrier protein] + AMP + diphosphate
General information:
Sequence
0 MRTDLIKPLH VALLENATRF AGKPAFADDH RTVTYGDLEA RTRRLAGHLA GLGVRHGDRV
60 AICLGNRVST VESYFAILRA GAVGVPLNPG SATAELEHPL TDSGATVVVT DAAQAARLRL
120 APHVELLVTG DDVPEGAHSY DELALSEPAE PAADDLELDE PAWMFYTSGT TGRPKGVVST
180 QRNCLWSVAS CYVPFPGLSD QDRVLWPLPL FHSLSHIACV LSATVVGASV RIADGSSADD
240 VMRLIEAESS TFLAGVPTTY HHLVRAARQR GFSAPSLRIG LAGGAVLGAG LRSEFEETFG
300 VPLIDAYGST ETCGAITMNP PDGARVEGSC GLAVPGVDVR VVDPDTGLDV PAGEEGEVWV
360 SGPNVMLGYH NSPEATAAAM RDGWFRTGDL ARRDDAGYFT ICGRIKELII RGGANIHPGE
420 VEAVLRTVDG VADAAVGGVP HDTLGEVPVA YVIPGPTGFD PAALIEKCRE QLSAYKVPDR
480 ILEVAHIPRT ASGKIRRGLL TDEPAQLRYA ATEHEEQSRH ADESVAAALR ARLSGLDERA
540 QCELLEDLVR TQAADVLGQP VPDGRAFRDL GFTSLAIVEL RNRLTEHTGL WLPASAVFDH
600 PTPAALAARV RAELLGITQA VAEPVVAADP GEPIAIVGMA CRLPGGVASP EDLWRLVAER
660 VDAVSEFPGD RGWDLDSLID PDRERAGTSY VGQGGFLHDA GEFDAGFFGI SPREAVAMDP
720 QQRLLLETSW EALENAGVDP IALKGTDTGV FSGLMGQGYG SGAVAPELEG FVTTGVASSV
780 ASGRVSYVLG LEGPAVTVDT ACSSSLVAMH LAAQALRQGE CSMALAGGVT VMATPGSFVE
840 FSRQRALAPD GRCKAFAAAA DGTGWSEGVG VVVLERLSVA RERGHRILAV LRGSAVNQDG
900 ASNGLTAPNG LSQQRVIRRA LAAAGLAPSD VDVVEAHGTG TTLGDPIEAQ ALLATYGQER
960 KQPLWLGSLK SNIGHAQAAA GVAGVIKMVQ ALRHETLPPT LHVDKPTLEV DWSAGAIELL
1020 TEARAWPRNG RPRRAGVSSF GVSGTNAHLI LEEAPAEEPV AAPELPVVPL VVSARSTESL
1080 SGQAERLASL LEGDVSLTEV AGALVSRRAV LDERAVVVAG SREEAVTGLR ALNTAGSGTP
1140 GKVVWVFPGQ GTQWAGMGRE LLAESPVFAE RIAECAAALA PWIDWSLVDV LRGEGDLGRV
1200 DVLQPACFAV MVGLAAVWES VGVRPDAVVG HSQGEIAAAC VSGALSLEDA AKVVALRSQA
1260 IAAELSGRGG MASVALGEDD VVSRLVDGVE VAAVNGPSSV VIAGDAHALD ATLEILSGEG
1320 IRVRRVAVDY ASHTRHVEDI RDTLAETLAG ISAQAPAVPF YSTVTSEWVR DAGVLDGGYW
1380 YRNLRNQVRF GAAATALLEQ GHTVFVEVSA HPVTVQPLSE LTGDAIGTLR REDGGLRRLL
1440 ASMGELFVRG IDVDWTAMVP AAGWVDLPTY AFEHRHYWLE PAEPASAGDP LLGTVVSTPG
1500 SDRLTAVAQW SRRAQPWAVD GLVPNAALVE AAIRLGDLAG TPVVGELVVD APVVLPRRGS
1560 REVQLIVGEP GEQRRRPIEV FSREADEPWT RHAHGTLAPA AAAVPEPAAA GDATDVTVAG
1620 LRDADRYGIH PALLDAAVRT VVGDDLLPSV WTGVSLLASG ATAVTVTPTA TGLRLTDPAG
1680 QPVLTVESVR GTPFVAEQGT TDALFRVDWP EIPLPTAETA DFLPYEATSA EATLSALQAW
1740 LADPAETRLA VVTGDCTEPG AAAIWGLVRS AQSEHPGRIV LADLDDPAVL PAVVASGEPQ
1800 VRVRNGVASV PRLTRVTPRQ DARPLDPEGT VLITGGTGTL GALTARHLVT AHGVRHLVLV
1860 SRRGEAPELQ EELTALGASV AIAACDVADR AQLEAVLRAI PAEHPLTAVI HTAGVLDDGV
1920 VTELTPDRLA TVRRPKVDAA RLLDELTREA DLAAFVLFSS AAGVLGNPGQ AGYAAANAEL
1980 DALARQRNSL DLPAVSIAWG YWATVSGMTE HLGDADLRRN QRIGMSGLPA DEGMALLDAA
2040 IATGGTLVAA KFDVAALRAT AKAGGPVPPL LRGLAPLPRR AAAKTASLTE RLAGLAETEQ
2100 AAALLDLVRR HAAEVLGHSG AESVHSGRTF KDAGFDSLTA VELRNRLAAA TGLTLSPAMI
2160 FDYPKPPALA DHLRAKLFGS AANRPAEIGT AAAEEPIAIV AMACRFPGGV HSPEDLWRLV
2220 ADGADAVTEF PADRGWDTDR LYHEDPDHEG TTYVRHGAFL DDAAGFDAAF FGISPNEALA
2280 MDPQQRLLLE TSWELFERAA IDPTTLAGQD IGVFAGVNSH DYSMRMHRAA GVEGFRLTGG
2340 SASVLSGRVA YHFGVEGPAV TVDTACSSSL VALHMAVQAL QRGECSMALA GGVMVMGTVE
2400 TFVEFSRQRG LAPDGRCKAF ADGADGTGWS EGVGLLLVER LSEAQRRGHQ VLAVVRGSAV
2460 NSDGASNGLT APNGPSQQRV IRKALAAAGL STSDVDAVEA HGTGTTLGDP IEAEALLATY
2520 GQNRETPLWL GSVKSNLGHT QAAAGVAGVI KMVMAMRHGV LPRTLHVDRP SSYVDWSAGA
2580 VELLTEARDW VSNGHPRRAG VSSFGIGGTN AHVVLEEVAA PITTPQPEPA EFLVPVLVSA
2640 RTAAGLRGQA GRLAAFLGDR TDVRVPDAAY ALATTRAQLD HRAVVLASDR AQLCADLAAF
2700 GSGVVTGTPV DGKLAVLFTG QGSQWAGMGR ELAETFPVFR DAFEAACEAV DTHLRERPLR
2760 EVVFDDSALL DQTMYTQGAL FAVETALFRL FESWGVRPGL LAGHSIGELA AAHVSGVLDL
2820 ADAGELVAAR GRLMQALPAG GAMVAVQATE DEVAPLLDGT VCVAAVNGPD SVVLSGTEAA
2880 VLAVADELAG RGRKTRRLAV SHAFHSPLME PMLDDFRAVA ERLTYRAGSL PVVSTLTGEL
2940 AALDSPDYWV GQVRNAVRFS DAVTALGAQG ASTFLELGPG GALAAMALGT LGGPEQSCVA
3000 TLRKNGAEVP DVLTALAELH VRGVGVDWTT VLDEPATAVG TVLPTYAFQH QRFWVDVDET
3060 AAVSVTPPPA EPIVDRPVQD VLELVRESAA VVLGHRDAGS FDLDRSFKDH GFDSLSAVKL
3120 RNRLRDFTGV ELPSTLIFDY PNPAVLADHL RAELLGERPA APAPVTRDVS DEPIAIVGMS
3180 TRLPGGADSP EELWKLVAEG RDAVSGFPVD RGWDLDGLYH PDPAHAGTSY TRSGGFLHDA
3240 AQFDAGLFGI SPREALAMDP QQRLLLETSW EALERAGVDP LSARGSDVGV FTGIVHHDYV
3300 TRLREVPEDV QGYTMTGTAS SVASGRVAYV FGFEGPAVTV DTACSSSLVA MHLAAQALRQ
3360 GECSMALAGG ATVMASPDAF LEFSRQRGLS ADGRCKAYAE GADGTGWAEG VGVVVLERLS
3420 VARERGHRVL AVLRGSAVNQ DGASNGLTAP NGPSQQRVIR GALASAGLAP SDVDVVEGHG
3480 TGTALGDPIE VQALLATYGQ EREQPLWLGS LKSNLGHTQA AAGVVGVIKM IMAMRHGVMP
3540 ATLHVDERTS QVDWSAGAIE VLTEAREWPR TGRPRRAGVS SFGASGTNAH LIIEEGPAEE
3600 AVDEEVASVV PLVVSARSAG SLAGQAGRLA AVLENESLAG VAGALVSGRA TLNERAVVIA
3660 GSRDEAQDGL QALARGENAP GVVTGTAGKP GKVVWVFPGQ GSQWMGMGRD LLDSSPVFAA
3720 RIKECAAALE QWTDWSLLDV LRGDADLLDR VDVVQPASFA MMVGLAAVWT SLGVTPDAVL
3780 GHSQGEIAAA CVSGALSLDD AAKVVALRSQ AIAGELAGRG GMASVALSEE DAVARLTPWA
3840 NRVEVAAVNS PSSVVIAGDA QALDEALEAL AGDGVRVRRV AVDYASHTRH VEAIAETLAK
3900 TLAGIDARVP AIPFYSTVLG TWIEQAVVDA GYWYRNLRQQ VRFGPSVADL AGLGHTVFVE
3960 ISAHPVLVQP LSEISDDAVV TGSLRRDDGG LRRLLASAAE LYVRGVAVDW TAAVPAAGWV
4020 DLPTYAFDRR HFWLHEAETA EAAEGMDGEF WTAIEQSDVD SLAELLELVP EQRGALSTVV
4080 PVLAQWRDRR RERSTAEKLR YQVTWQPLER EAAGVPGGRW LAVVPAGTTD ALLKELTGQG
4140 LDIVRLEIEE ASRAQLAEQL RNVLAEHDLT GVLSLLALDG GPADAAEITA STLALVQALG
4200 DTTTSAPLWC LTSGAVNIGI QDAVTAPAQA AVWGLGRAVA LERLDRWGGL VDLPAAIDAR
4260 TAQALLGVLN GAAGEDQLAV RRSGVYRRRL VRKPVPESAT SRWEPRGTVL VTGGAEGLGR
4320 HASVWLAQSG AERLIVTGTD GVDELTAELA EFGTTVEFCA DTDRDAIAQL VADSEVTAVV
4380 HAADIAQTSS VDDTGVADLD EVFAAKVTTA VWLDQLFEDT PLDAFVVFSS IAGIWGGGGQ
4440 GPAGAANAVL DALVEWRRAR GLKATSIAWG ALDQIGIGMD EAALAQLRRR GVIPMAPPLA
4500 VTAMVQAVAG NEKAVAVADM DWAAFIPAFT SVRPSPLFAD LPEAKAILRA AQDDGEDGDT
4560 ASSLADSLRA VPDAEQNRIL LKLVRGHAST VLGHSGAEGI GPRQAFQEVG FDSLAAVNLR
4620 NSLHAATGLR LPATLIFDYP TPEALVGYLR VELLREADDG LDGREDDLRR VLAAVPFARF
4680 KEAGVLDTLL GLADTGTEPG TDAETTEAAP AADDAELIDA LDISGLVQRA LGQTS
Download this sequence
in fasta formatDownload all sequences for 6.2.1.74
in fasta format
in csv (Excel, OpenOffice) formatSequence related references
Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
231093074
August P.R.,Tang L.,Yoon Y.J.,Ning S.,Mueller R.,Yu T.W.,Taylor M.,Hoffmann D.,Kim C.G.,Zhang X.,Hutchinson C.R.,Floss H.G.
Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699.
Chem. Biol.
5
69-79
1998
231093075
Kim C.G.,Yu T.W.,Fryhle C.B.,Handa S.,Floss H.G.
3-amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the formation of the precursor of mC7N units in rifamycin and related antibiotics.
J. Biol. Chem.
273
6030-6040
1998
231093077
Yu T.W.,Muller R.,Muller M.,Zhang X.,Draeger G.,Kim C.G.,Leistner E.,Floss H.G.
Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei S699.
J. Biol. Chem.
276
12546-12555
2001
