Sequence of PHYSA_PHYPO
EC Number:3.4.21.103
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
Reaction
Milk clotting activity. Preferential cleavage of Gly8-/-Ser in B chain of insulin most rapidly, followed by Leu11!Val, Cys(SO3H)19-/-Gly and Phe24-/-Phe. No action on Ac-Phe-Tyr(I)2.
Other sequences found for EC No. 3.4.21.103
General information:
Sequence
0 MRLLSLLFLL GLATLSFAVR SQWAQQGRAT HEALITIRFA LTQQNLDVLE RTLLDISDTT
60 SKNYGKWKTA EEVTELVAPA REISERVASF LERQGATKVE NFRDMVKVTA PVSWIEETLH
120 TNLFFFQHKT RTSKVIIRAD GGYKIPAEIA EHVDFVAGLF EFPSIKNART QVGAGVDGYI
180 VPYVIFDLYG IPTTFPVHPN SSICLVEFQD DQSYNKDDLK KFAKENEITE TVVSHTVGPY
240 SGSSADTEST LDVQYGGAIA LNTTVWFWTV EDWMYDFATD FLNTKNPPLV VSMSWGWPEP
300 EQCQVGNCTG DETSLEYVVR TNVEFQKIGA IGTTLLAASG DQGAPGDSDP ECNSKKKPLS
360 SIFPGASPWV LSVGATMLSN MTTEDADPSA EPPICKSWTC STSTTELVCT IPQALITTGG
420 GFSDYSLQPS YQNAAVAAYF KSGVPLPPQT DFNASNRGFP DVSALGHNYL IALSGDFEQV
480 DGTSASTPVF AAIIAHLNSY RLNNGKPPLA FAVPLIYQAF ASDPTIFNDI TTGDNKCTED
540 CCSKFGYEAT KGWDPVTGVG TPVFSKLLAF VQTLP
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
1229661
Nishii W.,Ueki T.,Miyashita R.,Kojima M.,Kim Y.-T.,Sasaki N.,Murakami-Murofushi K.,Takahashi K.
Structural and enzymatic characterization of physarolisin (formerly physaropepsin) proves that it is a unique serine-carboxyl proteinase.
Biochem. Biophys. Res. Commun.
301
1023-1029
2003
1229662
Murakami-Murofushi K.,Takahashi T.,Minowa Y.,Iino S.,Takeuchi T.,Kitagaki-Ogawa H.,Murofushi H.,Takahashi K.
Purification and characterization of a novel intracellular acid proteinase from the plasmodia of a true slime mold, Physarum polycephalum.
J. Biol. Chem.
265
19898-19903
1990
