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Sequence of HMNGT_SORBI

EC Number:2.4.1.85

EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
cyanohydrin beta-glucosyltransferase
Q9SBL1
Sorghum bicolor
492
52916
Reaction
UDP-alpha-D-glucose + (S)-4-hydroxymandelonitrile = UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside
Other sequences found for EC No. 2.4.1.85

General information:

Sequence
  0 MGSNAPPPPT PHVVLVPFPG QGHVAPLMQL ARLLHARGAR VTFVYTQYNY RRLLRAKGEA
 60 AVRPPATSSA RFRIEVIDDG LSLSVPQNDV GGLVDSLRKN CLHPFRALLR RLGQEVEGQD
120 APPVTCVVGD VVMTFAAAAA REAGIPEVQF FTASACGLLG YLHYGELVER GLVPFRDASL
180 LADDDYLDTP LEWVPGMSHM RLRDMPTFCR TTDPDDVMVS ATLQQMESAA GSKALILNTL
240 YELEKDVVDA LAAFFPPIYT VGPLAEVIAS SDSASAGLAA MDISIWQEDT RCLSWLDGKP
300 AGSVVYVNFG SMAVMTAAQA REFALGLASC GSPFLWVKRP DVVEGEEVLL PEALLDEVAR
360 GRGLVVPWCP QAAVLKHAAV GLFVSHCGWN SLLEATAAGQ PVLAWPCHGE QTTNCRQLCE
420 VWGNGAQLPR EVESGAVARL VREMMVGDLG KEKRAKAAEW KAAAEAAARK GGASWRNVER
480 VVNDLLLVGG KQ
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Sequence related references
Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
938545
Jones P.R.,Moeller B.L.,Hoej P.B.
The UDP-glucose:p-hydroxymandelonitrile-O-glucosyltransferase that catalyzes the last step in synthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor. Isolation, cloning, heterologous expression, and substrate specificity.
J. Biol. Chem.
274
35483-35491
1999
938546
Tattersall D.B.,Bak S.,Jones P.R.,Olsen C.E.,Nielsen J.K.,Hansen M.L.,Hoej P.B.,Moeller B.L.
Resistance to an herbivore through engineered cyanogenic glucoside synthesis.
Science
293
1826-1828
2001
938547
Hansen K.S.,Kristensen C.,Tattersall D.B.,Jones P.R.,Olsen C.E.,Bak S.,Moeller B.L.
The in vitro substrate regiospecificity of recombinant UGT85B1, the cyanohydrin glucosyltransferase from Sorghum bicolor.
Phytochemistry
64
143-151
2003
938548
Thorsoee K.S.,Bak S.,Olsen C.E.,Imberty A.,Breton C.,Moeller B.L.
Determination of catalytic key amino acids and UDP sugar donor specificity of the cyanohydrin glycosyltransferase UGT85B1 from Sorghum bicolor. Molecular modeling substantiated by site-specific mutagenesis and biochemical analyses.
Plant Physiol.
139
664-673
2005
938549
Kristensen C.,Morant M.,Olsen C.E.,Ekstroem C.T.,Galbraith D.W.,Moeller B.L.,Bak S.
Metabolic engineering of dhurrin in transgenic Arabidopsis plants with marginal inadvertent effects on the metabolome and transcriptome.
Proc. Natl. Acad. Sci. U.S.A.
102
1779-1784
2005
938550
Nielsen K.A.,Tattersall D.B.,Jones P.R.,Moeller B.L.
Metabolon formation in dhurrin biosynthesis.
Phytochemistry
69
88-98
2008