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Sequence of AROC_ECOLI

EC Number:4.2.3.5

EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
chorismate synthase
P12008
Escherichia coli (strain K12)
361
39137
Reaction
5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate
Other sequences found for EC No. 4.2.3.5

General information:

Sequence
show sequence in fasta format
  0 MAGNTIGQLF RVTTFGESHG LALGCIVDGV PPGIPLTEAD LQHDLDRRRP GTSRYTTQRR
 60 EPDQVKILSG VFEGVTTGTS IGLLIENTDQ RSQDYSAIKD VFRPGHADYT YEQKYGLRDY
120 RGGGRSSARE TAMRVAAGAI AKKYLAEKFG IEIRGCLTQM GDIPLDIKDW SQVEQNPFFC
180 PDPDKIDALD ELMRALKKEG DSIGAKVTVV ASGVPAGLGE PVFDRLDADI AHALMSINAV
240 KGVEIGDGFD VVALRGSQNR DEITKDGFQS NHAGGILGGI SSGQQIIAHM ALKPTSSITV
300 PGRTINRFGE EVEMITKGRH DPCVGIRAVP IAEAMLAIVL MDHLLRQRAQ NADVKTDIPR
360 W
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Sequence related references
Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
770130
Charles I.G.,Lamb H.K.,Pickard D.,Dougan G.,Hawkins A.R.
Isolation, characterization and nucleotide sequences of the aroC genes encoding chorismate synthase from Salmonella typhi and Escherichia coli.
J. Gen. Microbiol.
136
353-358
1990
770131
White P.J.,Millar G.,Coggins J.R.
The overexpression, purification and complete amino acid sequence of chorismate synthase from Escherichia coli K12 and its comparison with the enzyme from Neurospora crassa.
Biochem. J.
251
313-322
1988
770132
Yamamoto Y.,Aiba H.,Baba T.,Hayashi K.,Inada T.,Isono K.,Itoh T.,Kimura S.,Kitagawa M.,Makino K.,Miki T.,Mitsuhashi N.,Mizobuchi K.,Mori H.,Nakade S.,Nakamura Y.,Nashimoto H.,Oshima T.,Oyama S.,Saito N.,Sampei G.,Satoh Y.,Sivasundaram S.,Tagami H.,Takahashi H.,Takeda J.,Takemoto K.,Uehara K.,Wada C.,Yamagata S.,Horiuchi T.
Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.
DNA Res.
4
91-113
1997
770133
Blattner F.R.,Plunkett G. III,Bloch C.A.,Perna N.T.,Burland V.,Riley M.,Collado-Vides J.,Glasner J.D.,Rode C.K.,Mayhew G.F.,Gregor J.,Davis N.W.,Kirkpatrick H.A.,Goeden M.A.,Rose D.J.,Mau B.,Shao Y.
The complete genome sequence of Escherichia coli K-12.
Science
277
1453-1462
1997
770134
Hayashi K.,Morooka N.,Yamamoto Y.,Fujita K.,Isono K.,Choi S.,Ohtsubo E.,Baba T.,Wanner B.L.,Mori H.,Horiuchi T.
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.
Mol. Syst. Biol.
2
0-0
2006
770135
Ramjee M.K.,Coggins J.R.,Thorneley R.N.
A continuous, anaerobic spectrophotometric assay for chorismate synthase activity that utilizes photoreduced flavin mononucleotide.
Anal. Biochem.
220
137-141
1994
770136
Bornemann S.,Balasubramanian S.,Coggins J.R.,Abell C.,Lowe D.J.,Thorneley R.N.
Escherichia coli chorismate synthase: a deuterium kinetic-isotope effect under single-turnover and steady-state conditions shows that a flavin intermediate forms before the C-(6proR)-H bond is cleaved.
Biochem. J.
305
707-710
1995
770137
Bornemann S.,Lowe D.J.,Thorneley R.N.
The transient kinetics of Escherichia coli chorismate synthase: substrate consumption, product formation, phosphate dissociation, and characterization of a flavin intermediate.
Biochemistry
35
9907-9916
1996
770138
Macheroux P.,Bornemann S.,Ghisla S.,Thorneley R.N.
Studies with flavin analogs provide evidence that a protonated reduced FMN is the substrate-induced transient intermediate in the reaction of Escherichia coli chorismate synthase.
J. Biol. Chem.
271
25850-25858
1996
770139
Macheroux P.,Schoenbrunn E.,Svergun D.I.,Volkov V.V.,Koch M.H.,Bornemann S.,Thorneley R.N.
Evidence for a major structural change in Escherichia coli chorismate synthase induced by flavin and substrate binding.
Biochem. J.
335
319-327
1998
770140
Bornemann S.,Theoclitou M.E.,Brune M.,Webb M.R.,Thorneley R.N.,Abell C.
A secondary beta deuterium kinetic isotope effect in the chorismate synthase reaction.
Bioorg. Chem.
28
191-204
2000
770141
Osborne A.,Thorneley R.N.,Abell C.,Bornemann S.
Studies with substrate and cofactor analogues provide evidence for a radical mechanism in the chorismate synthase reaction.
J. Biol. Chem.
275
35825-35830
2000