Sequence of CP51_YEAST
EC Number:1.14.14.154
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
sterol 14alpha-demethylase
P10614
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
530
60720
Reaction
a 14alpha-formylsteroid + [reduced NADPH-hemoprotein reductase] + O2 = a DELTA14-steroid + formate + [oxidized NADPH-hemoprotein reductase] + H2O
Other sequences found for EC No. 1.14.14.154
General information:
Sequence
0 MSATKSIVGE ALEYVNIGLS HFLALPLAQR ISLIIIIPFI YNIVWQLLYS LRKDRPPLVF
60 YWIPWVGSAV VYGMKPYEFF EECQKKYGDI FSFVLLGRVM TVYLGPKGHE FVFNAKLADV
120 SAEAAYAHLT TPVFGKGVIY DCPNSRLMEQ KKFVKGALTK EAFKSYVPLI AEEVYKYFRD
180 SKNFRLNERT TGTIDVMVTQ PEMTIFTASR SLLGKEMRAK LDTDFAYLYS DLDKGFTPIN
240 FVFPNLPLEH YRKRDHAQKA ISGTYMSLIK ERRKNNDIQD RDLIDSLMKN STYKDGVKMT
300 DQEIANLLIG VLMGGQHTSA ATSAWILLHL AERPDVQQEL YEEQMRVLDG GKKELTYDLL
360 QEMPLLNQTI KETLRMHHPL HSLFRKVMKD MHVPNTSYVI PAGYHVLVSP GYTHLRDEYF
420 PNAHQFNIHR WNKDSASSYS VGEEVDYGFG AISKGVSSPY LPFGGGRHRC IGEHFAYCQL
480 GVLMSIFIRT LKWHYPEGKT VPPPDFTSMV TLPTGPAKII WEKRNPEQKI
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Sequence related references
Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
657632
Kalb V.F.,Woods C.W.,Turi T.G.,Dey C.R.,Sutter T.R.,Loper J.C.
Primary structure of the P450 lanosterol demethylase gene from Saccharomyces cerevisiae.
DNA
6
529-537
1987
657633
Ishida N.,Aoyama Y.,Hatanaka R.,Oyama Y.,Imajo S.,Ishiguro M.,Oshima T.,Nakazato H.,Noguchi T.,Maitra U.S.,Mohan V.P.,Sprinson D.B.,Yoshida Y.
A single amino acid substitution converts cytochrome P450(14DM) to an inactive form, cytochrome P450SG1: complete primary structures deduced from cloned DNAS.
Biochem. Biophys. Res. Commun.
155
317-323
1988
657634
Johnston M.,Andrews S.,Brinkman R.,Cooper J.,Ding H.,Dover J.,Du Z.,Favello A.,Fulton L.,Gattung S.,Geisel C.,Kirsten J.,Kucaba T.,Hillier L.W.,Jier M.,Johnston L.,Langston Y.,Latreille P.,Louis E.J.,Macri C.,Mardis E.,Menezes S.,Mouser L.,Nhan M.,Rifkin L.,Riles L.,St Peter H.,Trevaskis E.,Vaughan K.,Vignati D.,Wilcox L.,Wohldman P.,Waterston R.,Wilson R.,Vaudin M.
Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science
265
2077-2082
1994
657635
Engel S.R.,Dietrich F.S.,Fisk D.G.,Binkley G.,Balakrishnan R.,Costanzo M.C.,Dwight S.S.,Hitz B.C.,Karra K.,Nash R.S.,Weng S.,Wong E.D.,Lloyd P.,Skrzypek M.S.,Miyasato S.R.,Simison M.,Cherry J.M.
The reference genome sequence of Saccharomyces cerevisiae: Then and now.
G3 (Bethesda)
4
389-398
2014
657636
Kalb V.F.,Loper J.C.,Dey C.R.,Woods C.W.,Sutter T.R.
Isolation of a cytochrome P-450 structural gene from Saccharomyces cerevisiae.
Gene
45
237-245
1986
657637
Ohba M.,Sato R.,Yoshida Y.,Nishino T.,Katsuki H.
Involvement of cytochrome P-450 and a cyanide-sensitive enzyme in different steps of lanosterol demethylation by yeast microsomes.
Biochem. Biophys. Res. Commun.
85
21-27
1978
657638
Aoyama Y.,Yoshida Y.
The 14alpha-demethylation of lanosterol by a reconstituted cytochrome P-450 system from yeast microsomes.
Biochem. Biophys. Res. Commun.
85
28-34
1978
657639
Turi T.G.,Loper J.C.
Multiple regulatory elements control expression of the gene encoding the Saccharomyces cerevisiae cytochrome P450, lanosterol 14 alpha-demethylase (ERG11).
J. Biol. Chem.
267
2046-2056
1992
657640
Ghaemmaghami S.,Huh W.-K.,Bower K.,Howson R.W.,Belle A.,Dephoure N.,O'Shea E.K.,Weissman J.S.
Global analysis of protein expression in yeast.
Nature
425
737-741
2003
657641
Mo C.,Bard M.
Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex.
J. Lipid Res.
46
1991-1998
2005
657642
Kim H.,Melen K.,Oesterberg M.,von Heijne G.
A global topology map of the Saccharomyces cerevisiae membrane proteome.
Proc. Natl. Acad. Sci. U.S.A.
103
11142-11147
2006
657643
Li X.,Gerber S.A.,Rudner A.D.,Beausoleil S.A.,Haas W.,Villen J.,Elias J.E.,Gygi S.P.
Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.
J. Proteome Res.
6
1190-1197
2007
657644
Starita L.M.,Lo R.S.,Eng J.K.,von Haller P.D.,Fields S.
Sites of ubiquitin attachment in Saccharomyces cerevisiae.
Proteomics
12
236-240
2012
657645
Jorda T.,Puig S.
Regulation of ergosterol biosynthesis in Saccharomyces cerevisiae.
Genes (Basel)
11
0-0
2020
657646
Monk B.C.,Tomasiak T.M.,Keniya M.V.,Huschmann F.U.,Tyndall J.D.,O'Connell J.D. III,Cannon R.D.,McDonald J.G.,Rodriguez A.,Finer-Moore J.S.,Stroud R.M.
Architecture of a single membrane spanning cytochrome P450 suggests constraints that orient the catalytic domain relative to a bilayer.
Proc. Natl. Acad. Sci. U.S.A.
111
3865-3870
2014