Sequence of DIDH_RAT
EC Number:1.1.1.50
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
Reaction
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+
General information:
Sequence
0 MDSISLRVAL NDGNFIPVLG FGTTVPEKVA KDEVIKATKI AIDNGFRHFD SAYLYEVEEE
60 VGQAIRSKIE DGTVKREDIF YTSKLWSTFH RPELVRTCLE KTLKSTQLDY VDLYIIHFPM
120 ALQPGDIFFP RDEHGKLLFE TVDICDTWEA MEKCKDAGLA KSIGVSNFNC RQLERILNKP
180 GLKYKPVCNQ VECHLYLNQS KMLDYCKSKD IILVSYCTLG SSRDKTWVDQ KSPVLLDDPV
240 LCAIAKKYKQ TPALVALRYQ LQRGVVPLIR SFNAKRIKEL TQVFEFQLAS EDMKALDGLN
300 RNFRYNNAKY FDDHPNHPFT DE
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
466318
Pawlowski J.E.,Huizinga M.,Penning T.M.
Cloning and sequencing of the cDNA for rat liver 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase.
J. Biol. Chem.
266
8820-8825
1991
466319
Stolz A.,Rahimi-Kiani M.,Ameis D.,Chan E.,Ronk M.,Shively J.E.
Molecular structure of rat hepatic 3 alpha-hydroxysteroid dehydrogenase. A member of the oxidoreductase gene family.
J. Biol. Chem.
266
15253-15257
1991
466320
Cheng K.-C.,White P.C.,Qin K.-N.
Molecular cloning and expression of rat liver 3 alpha-hydroxysteroid dehydrogenase.
Mol. Endocrinol.
5
823-828
1991
466321
Usui E.,Okuda K.,Kato Y.,Noshiro M.
Rat hepatic 3 alpha-hydroxysteroid dehydrogenase: expression of cDNA and physiological function in bile acid biosynthetic pathway.
J. Biochem.
115
230-237
1994
466322
Lin H.K.,Hung C.F.,Moore M.,Penning T.M.
Genomic structure of rat 3alpha-hydroxysteroid/dihydrodiol dehydrogenase (3alpha-HSD/DD, AKR1C9).
J. Steroid Biochem. Mol. Biol.
71
29-39
1999
466323
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
Genome Res.
14
2121-2127
2004
466324
Del Bello B.,Maellaro E.,Sugherini L.,Santucci A.,Comporti M.,Casini A.F.
Purification of NADPH-dependent dehydroascorbate reductase from rat liver and its identification with 3 alpha-hydroxysteroid dehydrogenase.
Biochem. J.
304
385-390
1994
466325
Pawlowski J.,Huizinga M.,Penning T.M.
Isolation and partial characterization of a full-length cDNA clone for 3 alpha-hydroxysteroid dehydrogenase: a potential target enzyme for nonsteroidal anti-inflammatory drugs.
Agents Actions
34
289-293
1991
466326
Penning T.M.,Abrams W.R.,Pawlowski J.E.
Affinity labeling of 3 alpha-hydroxysteroid dehydrogenase with 3 alpha-bromoacetoxyandrosterone and 11 alpha-bromoacetoxyprogesterone. Isolation and sequence of active site peptides containing reactive cysteines; sequence confirmation using nucleotide sequence from a cDNA clone.
J. Biol. Chem.
266
8826-8834
1991
466327
Khanna M.,Qin K.-N.,Cheng K.-C.
Distribution of 3 alpha-hydroxysteroid dehydrogenase in rat brain and molecular cloning of multiple cDNAs encoding structurally related proteins in humans.
J. Steroid Biochem. Mol. Biol.
53
41-46
1995
466328
Hoog S.S.,Pawlowski J.E.,Alzari P.M.,Penning T.M.,Lewis M.
Three-dimensional structure of rat liver 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase: a member of the aldo-keto reductase superfamily.
Proc. Natl. Acad. Sci. U.S.A.
91
2517-2521
1994
466329
Bennett M.J.,Schlegel B.P.,Jez J.M.,Penning T.M.,Lewis M.
Structure of 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase complexed with NADP+.
Biochemistry
35
10702-10711
1996
466330
Bennett M.J.,Albert R.H.,Jez J.M.,Ma H.,Penning T.M.,Lewis M.
Steroid recognition and regulation of hormone action: crystal structure of testosterone and NADP+ bound to 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase.
Structure
5
799-812
1997
