Sequence of CP51A_ASPFU
EC Number:1.14.14.154
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
sterol 14alpha-demethylase
Q4WNT5
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)
515
58068
Reaction
a 14alpha-formylsteroid + [reduced NADPH-hemoprotein reductase] + O2 = a DELTA14-steroid + formate + [oxidized NADPH-hemoprotein reductase] + H2O
Other sequences found for EC No. 1.14.14.154
General information:
Sequence
0 MVPMLWLTAY MAVAVLTAIL LNVVYQLFFR LWNRTEPPMV FHWVPYLGST ISYGIDPYKF
60 FFACREKYGD IFTFILLGQK TTVYLGVQGN EFILNGKLKD VNAEEVYSPL TTPVFGSDVV
120 YDCPNSKLME QKKFIKYGLT QSALESHVPL IEKEVLDYLR DSPNFQGSSG RVDISAAMAE
180 ITIFTAARAL QGQEVRSKLT AEFADLYHDL DKGFTPINFM LPWAPLPHNK KRDAAHARMR
240 SIYVDIITQR RLDGEKDSQK SDMIWNLMNC TYKNGQQVPD KEIAHMMITL LMAGQHSSSS
300 ISAWIMLRLA SQPKVLEELY QEQLANLGPA GPDGSLPPLQ YKDLDKLPFH QHVIRETLRI
360 HSSIHSIMRK VKSPLPVPGT PYMIPPGRVL LASPGVTALS DEHFPNAGCW DPHRWENQAT
420 KEQENDKVVD YGYGAVSKGT SSPYLPFGAG RHRCIGEKFA YVNLGVILAT IVRHLRLFNV
480 DGKKGVPETD YSSLFSGPMK PSIIGWEKRS KNTSK
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
529750
Nierman W.C.,Pain A.,Anderson M.J.,Wortman J.R.,Kim H.S.,Arroyo J.,Berriman M.,Abe K.,Archer D.B.,Bermejo C.,Bennett J.W.,Bowyer P.,Chen D.,Collins M.,Coulsen R.,Davies R.,Dyer P.S.,Farman M.L.,Fedorova N.,Fedorova N.D.,Feldblyum T.V.,Fischer R.,Fosker N.,Fraser A.,Garcia J.L.,Garcia M.J.,Goble A.,Goldman G.H.,Gomi K.,Griffith-Jones S.,Gwilliam R.,Haas B.J.,Haas H.,Harris D.E.,Horiuchi H.,Huang J.,Humphray S.,Jimenez J.,Keller N.,Khouri H.,Kitamoto K.,Kobayashi T.,Konzack S.,Kulkarni R.,Kumagai T.,Lafton A.,Latge J.-P.,Li W.,Lord A.,Lu C.,Majoros W.H.,May G.S.,Miller B.L.,Mohamoud Y.,Molina M.,Monod M.,Mouyna I.,Mulligan S.,Murphy L.D.,O'Neil S.,Paulsen I.,Penalva M.A.,Pertea M.,Price C.,Pritchard B.L.,Quail M.A.,Rabbinowitsch E.,Rawlins N.,Rajandream M.A.,Reichard U.,Renauld H.,Robson G.D.,Rodriguez de Cordoba S.,Rodriguez-Pena J.M.,Ronning C.M.,Rutter S.,Salzberg S.L.,Sanchez M.,Sanchez-Ferrero J.C.,Saunders D.,Seeger K.,Squares R.,Squares S.,Takeuchi M.,Tekaia F.,Turner G.,Vazquez de Aldana C.R.,Weidman J.,White O.,Woodward J.R.,Yu J.-H.,Fraser C.M.,Galagan J.E.,Asai K.,Machida M.,Hall N.,Barrell B.G.,Denning D.W.
Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus.
Nature
438
1151-1156
2005
529751
Venkateswarlu K.,Kelly S.L.
Stereoselective interaction of SCH 39304, a triazole, with sterol 14alpha-demethylase of Aspergillus fumigatus.
J. Antimicrob. Chemother.
39
597-601
1997
529752
Mellado E.,Diaz-Guerra T.M.,Cuenca-Estrella M.,Rodriguez-Tudela J.L.
Identification of two different 14-alpha sterol demethylase-related genes (cyp51A and cyp51B) in Aspergillus fumigatus and other Aspergillus species.
J. Clin. Microbiol.
39
2431-2438
2001
529753
Mann P.A.,Parmegiani R.M.,Wei S.Q.,Mendrick C.A.,Li X.,Loebenberg D.,DiDomenico B.,Hare R.S.,Walker S.S.,McNicholas P.M.
Mutations in Aspergillus fumigatus resulting in reduced susceptibility to posaconazole appear to be restricted to a single amino acid in the cytochrome P450 14alpha-demethylase.
Antimicrob. Agents Chemother.
47
577-581
2003
529754
Diaz-Guerra T.M.,Mellado E.,Cuenca-Estrella M.,Rodriguez-Tudela J.L.
A point mutation in the 14alpha-sterol demethylase gene cyp51A contributes to itraconazole resistance in Aspergillus fumigatus.
Antimicrob. Agents Chemother.
47
1120-1124
2003
529755
Nascimento A.M.,Goldman G.H.,Park S.,Marras S.A.,Delmas G.,Oza U.,Lolans K.,Dudley M.N.,Mann P.A.,Perlin D.S.
Multiple resistance mechanisms among Aspergillus fumigatus mutants with high-level resistance to itraconazole.
Antimicrob. Agents Chemother.
47
1719-1726
2003
529756
Mellado E.,Garcia-Effron G.,Alcazar-Fuoli L.,Cuenca-Estrella M.,Rodriguez-Tudela J.L.
Substitutions at methionine 220 in the 14alpha-sterol demethylase (Cyp51A) of Aspergillus fumigatus are responsible for resistance in vitro to azole antifungal drugs.
Antimicrob. Agents Chemother.
48
2747-2750
2004
529757
Gollapudy R.,Ajmani S.,Kulkarni S.A.
Modeling and interactions of Aspergillus fumigatus lanosterol 14-alpha demethylase 'A' with azole antifungals.
Bioorg. Med. Chem.
12
2937-2950
2004
529758
Garcia-Effron G.,Mellado E.,Gomez-Lopez A.,Alcazar-Fuoli L.,Cuenca-Estrella M.,Rodriguez-Tudela J.L.
Differences in interactions between azole drugs related to modifications in the 14-alpha sterol demethylase gene (cyp51A) of Aspergillus fumigatus.
Antimicrob. Agents Chemother.
49
2119-2121
2005
529759
Mellado E.,Garcia-Effron G.,Buitrago M.J.,Alcazar-Fuoli L.,Cuenca-Estrella M.,Rodriguez-Tudela J.L.
Targeted gene disruption of the 14-alpha sterol demethylase (cyp51A) in Aspergillus fumigatus and its role in azole drug susceptibility.
Antimicrob. Agents Chemother.
49
2536-2538
2005
529760
Chen J.,Li H.,Li R.,Bu D.,Wan Z.
Mutations in the cyp51A gene and susceptibility to itraconazole in Aspergillus fumigatus serially isolated from a patient with lung aspergilloma.
J. Antimicrob. Chemother.
55
31-37
2005
529761
Balashov S.V.,Gardiner R.,Park S.,Perlin D.S.
Rapid, high-throughput, multiplex, real-time PCR for identification of mutations in the cyp51A gene of Aspergillus fumigatus that confer resistance to itraconazole.
J. Clin. Microbiol.
43
214-222
2005
529762
Trama J.P.,Mordechai E.,Adelson M.E.
Detection of Aspergillus fumigatus and a mutation that confers reduced susceptibility to itraconazole and posaconazole by real-time PCR and pyrosequencing.
J. Clin. Microbiol.
43
906-908
2005
529763
Ferreira M.E.,Colombo A.L.,Paulsen I.,Ren Q.,Wortman J.,Huang J.,Goldman M.H.,Goldman G.H.
The ergosterol biosynthesis pathway, transporter genes, and azole resistance in Aspergillus fumigatus.
Med. Mycol.
43
0-0
2005
529764
da Silva Ferreira M.E.,Malavazi I.,Savoldi M.,Brakhage A.A.,Goldman M.H.,Kim H.S.,Nierman W.C.,Goldman G.H.
Transcriptome analysis of Aspergillus fumigatus exposed to voriconazole.
Curr. Genet.
50
32-44
2006
529765
Mellado E.,Garcia-Effron G.,Alcazar-Fuoli L.,Melchers W.J.,Verweij P.E.,Cuenca-Estrella M.,Rodriguez-Tudela J.L.
A new Aspergillus fumigatus resistance mechanism conferring in vitro cross-resistance to azole antifungals involves a combination of cyp51A alterations.
Antimicrob. Agents Chemother.
51
1897-1904
2007
529766
Schrettl M.,Kim H.S.,Eisendle M.,Kragl C.,Nierman W.C.,Heinekamp T.,Werner E.R.,Jacobsen I.,Illmer P.,Yi H.,Brakhage A.A.,Haas H.
SreA-mediated iron regulation in Aspergillus fumigatus.
Mol. Microbiol.
70
27-43
2008
529767
Alcazar-Fuoli L.,Mellado E.,Garcia-Effron G.,Lopez J.F.,Grimalt J.O.,Cuenca-Estrella J.M.,Rodriguez-Tudela J.L.
Ergosterol biosynthesis pathway in Aspergillus fumigatus.
Steroids
73
339-347
2008
529768
Fraczek M.G.,Bromley M.,Bowyer P.
An improved model of the Aspergillus fumigatus CYP51A protein.
Antimicrob. Agents Chemother.
55
2483-2486
2011
529769
Snelders E.,Karawajczyk A.,Verhoeven R.J.,Venselaar H.,Schaftenaar G.,Verweij P.E.,Melchers W.J.
The structure-function relationship of the Aspergillus fumigatus cyp51A L98H conversion by site-directed mutagenesis: the mechanism of L98H azole resistance.
Fungal Genet. Biol.
48
1062-1070
2011
529770
Alcazar-Fuoli L.,Cuesta I.,Rodriguez-Tudela J.L.,Cuenca-Estrella M.,Sanglard D.,Mellado E.
Three-dimensional models of 14alpha-sterol demethylase (Cyp51A) from Aspergillus lentulus and Aspergillus fumigatus: an insight into differences in voriconazole interaction.
Int. J. Antimicrob. Agents
38
426-434
2011
529771
Blatzer M.,Barker B.M.,Willger S.D.,Beckmann N.,Blosser S.J.,Cornish E.J.,Mazurie A.,Grahl N.,Haas H.,Cramer R.A.
SREBP coordinates iron and ergosterol homeostasis to mediate triazole drug and hypoxia responses in the human fungal pathogen Aspergillus fumigatus.
PLoS Genet.
7
0-0
2011
529772
Blosser S.J.,Cramer R.A.
SREBP-dependent triazole susceptibility in Aspergillus fumigatus is mediated through direct transcriptional regulation of erg11A (cyp51A).
Antimicrob. Agents Chemother.
56
248-257
2012
529773
Warrilow A.G.,Parker J.E.,Price C.L.,Nes W.D.,Kelly S.L.,Kelly D.E.
In Vitro biochemical study of CYP51-mediated azole resistance in Aspergillus fumigatus.
Antimicrob. Agents Chemother.
59
7771-7778
2015
529774
Colley T.,Sehra G.,Chowdhary A.,Alanio A.,Kelly S.L.,Kizawa Y.,Armstrong-James D.,Fisher M.C.,Warrilow A.G.S.,Parker J.E.,Kelly D.E.,Kimura G.,Nishimoto Y.,Sunose M.,Onions S.,Crepin D.,Lagasse F.,Crittall M.,Shannon J.,McConville M.,King-Underwood J.,Naylor A.,Bretagne S.,Murray J.,Ito K.,Strong P.,Rapeport G.
In vitro and in vivo efficacy of a novel and long-acting fungicidal azole, PC1244, on Aspergillus fumigatus infection.
Antimicrob. Agents Chemother.
62
0-0
2018
