Sequence of ERYA3_SACER
EC Number:2.3.1.94
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
Reaction
propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH + 6 H+ = 6-deoxyerythronolide B + 7 CoA + 6 CO2 + H2O + 6 NADP+
Other sequences found for EC No. 2.3.1.94
General information:
Sequence
0 MSGDNGMTEE KLRRYLKRTV TELDSVTARL REVEHRAGEP IAIVGMACRF PGDVDSPESF
60 WEFVSGGGDA IAEAPADRGW EPDPDARLGG MLAAAGDFDA GFFGISPREA LAMDPQQRIM
120 LEISWEALER AGHDPVSLRG SATGVFTGVG TVDYGPRPDE APDEVLGYVG TGTASSVASG
180 RVAYCLGLEG PAMTVDTACS SGLTALHLAM ESLRRDECGL ALAGGVTVMS SPGAFTEFRS
240 QGGLAADGRC KPFSKAADGF GLAEGAGVLV LQRLSAARRE GRPVLAVLAG SAVNQDGASN
300 GLTAPSGPAQ QRVIRRALEN AGVRAGDVDY VEAHGTGTRL GDPIEVHALL STYGAERDPD
360 DPLWIGSVKS NIGHTQAAAG VAGVMKAVLA LRHGEMPRTL HFDEPSPQIE WDLGAVSVVS
420 QARSWPAGER PRRAGVSSFG ISGTNAHVIV EEAPEADEPE PAPDSGPVPL VLSGRDEQAM
480 RAQAGRLADH LAPEPRNSLR DTGFTLATRA SAMEHRAVVV GDRDEALAGL RAVADRRIAD
540 RTATGQGPNS PRRVAMVFPG QGAQWQGMAR DLLRESQVFA DSIRDCERAL APHVDWSLTD
600 LLSGARPLDR VDVVQPALFA VMVSLAALWR SHGVEPAAVV GHSQGEIAAA HVAGALTLED
660 AAKLVAVRSR VLRRLGGQGG MASFGLGTEQ AAERIGRFAG ALSIASVNGP RSVVVVAGES
720 GPLDELIAEC EAEAHKARRI PVDYASHSPQ VESLREELLT ELAGISPVSA DVALYSTTTG
780 QPIDTATMDT AYWYANLREQ VRFQDATRQL AEAGFDAFVE VSPHPVLTVG IEATLDSALP
840 ADAGACVVGT LRRDRGGLAD FHTALGEAYA QGVEVDWSPA FADARPVELP VYPFQRYWLP
900 IPTGGRARDE DDDWRYQVVW REAEWESASL AGRVLLVTGP GVPSELSDAI RSGLEQSGAT
960 VLTCDVESRS TIGTALEAAD TDALSTVGVA AVPHGEAVDP SLDALALVQA LGAAGVEAPL
1020 WVLTRNAVQV ADGELVDPAQ AMVGGLGRVV GIEQPGRWGG LVDLVDADAA SIRSLAAVLA
1080 DPRGEEQVAI RADGIKVARL VPAPARARTH PLEPLAGTVL VTGGTGGIGA HLARWLARSG
1140 AEHLVLLGRR GADAPGASEL REELTALGTG VTIAACDVAD RARLEAVLAA EAAAEGRTVS
1200 AVMHAAGVST STPLDDLTEA EFTEIADVKV RGTVNLDELC PDLDAFVLFS SNAGVWGSPG
1260 LASYAAANAF LDGFARAARS EGAPVTSIAW GLWAGQNMAG DEGGEYLRSQ GLRAMDPDRA
1320 VEELHITLDH GQTSVSVVDM DRRRFVELFT AARHRPLFDE IAGARAEARQ SEEGPALAQR
1380 LAALLCDGRE REHLAHLIRA EVAAVLGHGD DAAIDRDRAF RDLGFDSMTA VDLRNRLAAV
1440 TGVREAATVV FDHPTITRLA DHYLERLVGA AEAEQAPALV REVPPKDADD PIAIVGMACR
1500 FPGGVHNPGE LWEFIVGGGD AVTEMPTDRG WDLDALFDPD PQRHGTSYSR HGAFLDGAAD
1560 FDAAFFGISP REALAMDPQQ RQVLETTWEL FENAGIDPHS VRGSDTGVFL GAAYQGYGQD
1620 AVVPEDSEGY LLTGNSSAVV SGRVAYVLGL EGPAVTVDTA CSSSLVALHS ACGSLRDGDC
1680 GLAVAGGVSV MAGPEVFTEF SRQGGLAVDG RCKAFSAEAD GFGLPEGVAV VQLQRLSDGP
1740 AEGGRQVLGV VAGSAINQDG ATNGLAAPSG VAQQRVIRKA WARAGITGAD VAVVEAHGTG
1800 TRLGDPVEAS ALLATYGKSR GSSGPVLLGS VKSNIGHAQA AAGVAGVIKV VLGLNRGLVP
1860 PMLCRGERSP LIEWSSGGVE LAEAVSPWPP AADGVRRAGV SAFGVSGTNA HVIIAEPPEP
1920 EPLPEPGPVG VLAAANSVPV LLSARTETAL AAQARLLESA VDDSVPLTAL ASALATGRAH
1980 LPRRAALLAG DHEQLRGQLR AVAEGVAAPG ATTGTASAGG VVFVFPGQGA QWEGMARGLL
2040 SVPVFAESIA ECDAVLSEVA GFSASEVLEQ RPDAPSLERV DVVQPVLFSV MVSLARLWGA
2100 CGVSPSAVIG HSQGEIAAAV VAGVLSLEDG VRVVALRAKA LRALAGKGGM VSLAAPGERA
2160 RALIAPWEDR ISVAAVNSPS SVVVSGDPEA LAELVARCED EGVRAKTLPV DYASHSRHVE
2220 EIRETILADL DGISARRAAI PLYSTLHGER RDMGPRYWYD NLRSQVRFDE AVSAQSPDGH
2280 ATFVEMSPHP VLTAAVQEIA ADAVAIGSLH RDTAEEHLIA ELARAHVHGV AVDWRNVFPA
2340 APPVALPNYP FEPQRYWLAP EVSDQLADSR YRVDWRPLAT TPVDLEGGFL VHGSAPESLT
2400 SAVEKAGGVV PVASADREAL AAALREVPGE VAGVLSVHTG AANALALHQS LGEAGVRAPL
2460 WLVTSRAVAL GESEPVDPEQ AMVWGLGRVM GLETPERWGG LVDLPAEPAP GDGEAFVACL
2520 GADGHEDQVA IRDHARYGRR LVRAPLGTRE SSWEPAGTAL VTGGTGALGG HVARHLARCG
2580 VEDLVLVSRR GVDAPAAAEL EAELVALGPK TTITACDVAD REQLSKLLEE LRGQGRPVRT
2640 VVHTAGVPES RPLHEIGELE SVCAAKVTGA RLLDELCPDA ETFVLFSSGA GVWGSANLGA
2700 YSAANAYLDA LAHRRRAEGR AATSVAWGAW AGEGMATGDL EGLTRRGLRP MAPDRAIRAL
2760 HQALDNGDTC VSIADVDWEA FAVGFTAARP RPLLDELVTP AVGAVPAVQA APAREMTSQE
2820 LLEFTHSHVA AILGHSSPDA VGQDQPFTEL GFDSLTAVGL RNQLQQATGL ALPATLVFEH
2880 PTVRRLADHI GQQLDSGTPA REASSALRDG YRQAGVSGRV RSYLDLLAGL SDFREHFDGS
2940 DGFSLDLVDM ADGPGEVTVI CCAGTAAISG PHEFTRLAGA LRGIAPVRAV PQPGYEEGEP
3000 LPSSMAAVAA VQADAVIRTQ GDKPFVVAGH SAGALMAYAL ATELLDRGHP PRGVVLIDVY
3060 PPGHQDAMNA WLEELTATLF DRETVRMDDT RLTALGAYDR LTGQWRPRET GLPTLLVSAG
3120 EPMGPWPDDS WKPTWPFEHD TVAVPGDHFT MVQEHADAIA RHIDAWLGGG NS
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
795906
Cortes J.,Haydock S.F.,Roberts G.A.,Bevitt D.J.,Leadlay P.F.
An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea.
Nature
348
176-178
1990
795907
Donadio S.,Staver M.J.,McAlpine J.B.,Swanson S.J.,Katz L.
Modular organization of genes required for complex polyketide biosynthesis.
Science
252
675-679
1991
795908
Bevitt D.J.,Cortes J.,Haydock S.F.,Leadlay P.F.
6-deoxyerythronolide-B synthase 2 from Saccharopolyspora erythraea. Cloning of the structural gene, sequence analysis and inferred domain structure of the multifunctional enzyme.
Eur. J. Biochem.
204
39-49
1992
795909
Caffrey P.,Bevitt D.J.,Staunton J.,Leadlay P.F.
Identification of DEBS 1, DEBS 2 and DEBS 3, the multienzyme polypeptides of the erythromycin-producing polyketide synthase from Saccharopolyspora erythraea.
FEBS Lett.
304
225-228
1992
795910
Khosla C.,Tang Y.,Chen A.Y.,Schnarr N.A.,Cane D.E.
Structure and mechanism of the 6-deoxyerythronolide B synthase.
Annu. Rev. Biochem.
76
195-221
2007
795911
Zhang H.,Wang Y.,Wu J.,Skalina K.,Pfeifer B.A.
Complete biosynthesis of erythromycin A and designed analogs using E. coli as a heterologous host.
Chem. Biol.
17
1232-1240
2010
795912
Tsai S.-C.,Miercke L.J.W.,Krucinski J.,Gokhale R.,Chen J.C.-H.,Foster P.G.,Cane D.E.,Khosla C.,Stroud R.M.
Crystal structure of the macrocycle-forming thioesterase domain of the erythromycin polyketide synthase: versatility from a unique substrate channel.
Proc. Natl. Acad. Sci. U.S.A.
98
14808-14813
2001
795913
Tsai S.-C.,Lu H.,Cane D.E.,Khosla C.,Stroud R.M.
Insights into channel architecture and substrate specificity from crystal structures of two macrocycle-forming thioesterases of modular polyketide synthases.
Biochemistry
41
12598-12606
2002
795914
Tang Y.,Kim C.Y.,Mathews I.I.,Cane D.E.,Khosla C.
The 2.7-Angstrom crystal structure of a 194-kDa homodimeric fragment of the 6-deoxyerythronolide B synthase.
Proc. Natl. Acad. Sci. U.S.A.
103
11124-11129
2006
795915
Argyropoulos P.,Bergeret F.,Pardin C.,Reimer J.M.,Pinto A.,Boddy C.N.,Schmeing T.M.
Towards a characterization of the structural determinants of specificity in the macrocyclizing thioesterase for deoxyerythronolide B biosynthesis.
Biochim. Biophys. Acta
1860
486-497
2016
