EC Number   |
Protein Variants |
Reference |
|---|
    6.3.5.1 | C175S |
in contrast with the wild-type NADsyn1, the activity of the mutant NADsyn1 (C175S-NADsyn1) is not detected when glutamine is used as a substrate, whereas the activity remains unaltered with NH4Cl |
652364 |
| 6.3.5.1 | C176A |
activity smilar to wild-type |
705873 |
| 6.3.5.1 | C176A |
complete loss of glutamine-dependent activity, retains 90% of its NH3-dependent activity |
663373 |
| 6.3.5.1 | C176A |
site-directed mutagenesis, inactive mutant, analysis of ligand binding structures |
-, 726904 |
| 6.3.5.1 | D593A |
poor synthetase activity |
674687 |
| 6.3.5.1 | D593A/F622A |
synthetase dead |
674687 |
| 6.3.5.1 | D656A |
strong decrease in catalytic efficiency |
705873 |
| 6.3.5.1 | E177A |
poor synthetase activity |
674687 |
| 6.3.5.1 | E52A |
complete loss of glutamine-dependent activity, retains 30% of its NH3-dependent activity |
663373 |
| 6.3.5.1 | F622A |
poor synthetase activity |
674687 |
