EC Number   |
Protein Variants |
Reference |
|---|
    6.3.4.3 | E98D |
36% of wild-type activity |
649939 |
| 6.3.4.3 | E98Q |
93% of wild-type activity, slight increase in thermal stability in the absence of monovalent cation |
649939 |
| 6.3.4.3 | E98S |
61% of wild-type activity |
649939 |
| 6.3.4.3 | G1958A |
the mutation is not associated with depression in postmenopausal women |
693974 |
| 6.3.4.3 | H125S |
site-directed mutant enzymes H125S, H131S, and H268S do not fold properly and/or do not associate to the active tetramer and, as a consequence are susceptible to intracellular proteolytic digestion |
1524 |
| 6.3.4.3 | H131S |
site-directed mutant enzymes H125S, H131S, and H268S do not fold properly and/or do not associate to the active tetramer and, as a consequence are susceptible to intracellular proteolytic digestion |
1524 |
| 6.3.4.3 | H268S |
site-directed mutant enzymes H125S, H131S, and H268S do not fold properly and/or do not associate to the active tetramer and, as a consequence are susceptible to intracellular proteolytic digestion |
1524 |
| 6.3.4.3 | K386E |
the mutation completely abrogates synthetase activity |
727607 |
| 6.3.4.3 | more |
construction of a DCS disruption knockout mutant fibroblast SF6 cell line lacking the cytoplasmic isozyme but expressing active mitochondrial isozyme from a disruption mutant embryonic stem cell line |
662447 |
| 6.3.4.3 | more |
in Escherichia coli DELTAfolD mutants, the essential function of folD can be replaced by Clostridium perfringens fhs when it is provided on a medium-copy-number plasmid or integrated as a single-copy gene in the chromosome. The fhs-supported folD deletion (DELTAfolD) strain grows well in a complex medium. The DELTAfolD strain, harboring heterologous fhs on the chromosome, shows a high NADP+ -to-NADPH ratio and hypersensitivity to trimethoprim. The presence of fhs in Escherichia coli is disadvantageous for its aerobic growth. but under hypoxia, Escherichia coli strains harboring fhs outcompete those lacking it |
-, 742789 |
