EC Number   |
Protein Variants |
Reference |
|---|
   6.3.4.19 | D137A |
no activity detectable |
706481 |
| 6.3.4.19 | D191A |
no activity detectable |
706481 |
| 6.3.4.19 | D36A |
no activity detectable |
706481 |
| 6.3.4.19 | E140A |
Km-value for L-lysine is 2.2fold higher than wild-type value, Km-value for ATP is about 7fold higher than wild-type value, Km-value for [tRNAIle2]-cytidine34 is 4.7fold higher than wild-type value |
706481 |
| 6.3.4.19 | H133A |
Km-value for L-lysine is 1.6 fold higher than wild-type value, Km-value for ATP is 2.5fold lower than wild-type value, Km-value for [tRNAIle2]-cytidine34 is similar to wild-type value |
706481 |
| 6.3.4.19 | more |
a mutation study reveals that Escherichia coli TilS discriminates tRNAIle from the structurally similar tRNAMet having the same anticodon loop by recognizing the anticodon loop, the anticodon stem, and the acceptor stem |
705718 |
| 6.3.4.19 | N194A |
no activity detectable |
706481 |
| 6.3.4.19 | R113A |
no activity detectable |
706481 |
| 6.3.4.19 | R174A |
Km-value for L-lysine is 1.8fold higher than wild-type value, Km-value for ATP is 2.1fold higher than wild-type value, Km-value for [tRNAIle2]-cytidine34 is 6fold higher than wild-type value |
706481 |
| 6.3.4.19 | R201A |
no activity detectable |
706481 |
