EC Number   |
Protein Variants |
Reference |
|---|
    6.3.1.9 | C57A |
functional synthetase devoid of amidase activity. In the presence of inducer, this line showed decreased growth in vitro and decreased virulence in vivo, indicating that the amidase function is not absolutely required for viability |
705789 |
| 6.3.1.9 | C59A |
mutant lacks the amidase activity, while the synthetase activity is similar to wild-type. Constructed for the production of trypanothione and trypanothione disulfide in >200 mg quantities. The protocol also allows the synthesis of related glutathione conjugates |
704099 |
| 6.3.1.9 | DELTAN236 |
unable to produce trypanothione |
675869 |
| 6.3.1.9 | DELTAN250 |
unable to produce trypanothione |
675869 |
| 6.3.1.9 | more |
elimination of both LiTRYS alleles is not possible unless parasites are previously complemented with an episomal copy of the gene. Retention of extrachromosomal LiTRYS in the trys-/-/+TRYS line after several passages in culture |
-, 744964 |
| 6.3.1.9 | more |
enzyme downregulation leads to decreased growth rate of the parasite, increased H2O2 and tert-butylhydroperoxide sensitivity, enzyme knockdown leads to depletion of both trypanothione and glutathionylspermidine, and glutathione and polyamine accumulation, RNA interference method, overview |
661011 |
| 6.3.1.9 | more |
enzyme downregulation leads to increased trypanocidic drug sensitivity, enzyme knockdown leads to depletion of both trypanothione and glutathionylspermidine and glutathione accumulation, RNA interference method, overview |
661810 |
| 6.3.1.9 | more |
introduction of the trypanothione synthetase and reductase genes from Trypanosoma cruzi into Escherichia coli results in decreased intracellular levels of reactive oxygen species. Escherichia coli genomic DNA is protected from radiation-induced DNA damage by 4.6fold in the presence of trypanothione compared to control bacteria. The transgenic Escherichia coli expressing trypanothione are 4.3fold more resistant to killing by 137Cs gamma-radiation |
716890 |
| 6.3.1.9 | R553E |
site-directed mutagenesis |
662446 |
| 6.3.1.9 | R553K |
site-directed mutagenesis |
662446 |
