EC Number   |
Protein Variants |
Reference |
|---|
    6.3.1.8 | C338A |
increased Km compared to the wild type enzyme |
673330 |
| 6.3.1.8 | C79A |
mutant retains synthetase activity, but loses amidase activity |
649733 |
| 6.3.1.8 | E391A |
increased Km compared to the wild type enzyme |
673330 |
| 6.3.1.8 | E392A |
increased Km compared to the wild type enzyme |
673330 |
| 6.3.1.8 | K607A |
increased Km compared to the wild type enzyme |
673330 |
| 6.3.1.8 | more |
enzyme downregulation leads to decreased growth rate of the parasite, increased H2O2 and tert-butylhydroperoxide sensitivity, enzyme knockdown leads to depletion of both trypanothione and glutathionylspermidine, and glutathione and polyamine accumulation, RNA interference method, overview |
661011 |
| 6.3.1.8 | more |
enzyme downregulation leads to increased trypanocidic drug sensitivity, enzyme knockdown leads to depletion of both trypanothione and glutathionylspermidine and glutathione accumulation, RNA interference method, overview |
661810 |
| 6.3.1.8 | more |
generation of a knockout GSPS mutant line by gene targeting, the mutant is viable and capable of replicating in both life cycle stages of the parasite, the extracellular promastigotes residing in the insect vector and the intracellular amastigotes thriving in mammalian hosts |
-, 744964 |
| 6.3.1.8 | R316E |
no activity |
673330 |
| 6.3.1.8 | R538A |
increased Km compared to the wild type enzyme |
673330 |
