EC Number |
Protein Variants |
Reference |
---|
6.3.1.19 | A196S |
site-directed mutagenesis, the mutant shows increased pupylation activity with substrate PanB compared to wild-type |
745553 |
6.3.1.19 | D208A |
site-directed mutagenesis, the mutant shows reduced pupylation activity with substrate PanB compared to wild-type |
-, 745553 |
6.3.1.19 | D64N |
site-directed mutagenesis, a catalytically inactive mutant |
-, 745553 |
6.3.1.19 | E209A |
site-directed mutagenesis, the mutant shows highly reduced pupylation activity with substrate PanB compared to wild-type |
745553 |
6.3.1.19 | E70A |
site-directed mutagenesis, a catalytically inactive mutant |
745553 |
6.3.1.19 | E9A |
site-directed mutagenesis, the PafA mutant without ligase activity cannot be pupylated |
-, 746202 |
6.3.1.19 | K31R |
site-directed mutagenesis, the mutation attenuates the poly-pupylation of PafA |
746202 |
6.3.1.19 | K320R |
site-directed mutagenesis, the self-pupylation of PafA K320R mutant is not totally eliminated, suggesting that PafA can be poorly modified by Pup on other lysine residue(s) besides K320 in the in vitro reaction system. Wild-type PafA is less stable than PafA K320R, which cannot be pupylated in vivo |
-, 746202 |
6.3.1.19 | K61R |
site-directed mutagenesis, the mutation attenuates the poly-pupylation of PafA |
-, 746202 |
6.3.1.19 | K7R |
site-directed mutagenesis, the mutation attenuates the poly-pupylation of PafA |
-, 746202 |