EC Number |
Protein Variants |
Reference |
---|
6.2.1.3 | C343S |
no luminescence enhancement |
697221 |
6.2.1.3 | C343S/G344T |
no luminescence enhancement |
697221 |
6.2.1.3 | C343S/G344T/L345S |
increase of luminescence activity to 100% |
697221 |
6.2.1.3 | C343S/L345S |
increase of luminescence activity |
697221 |
6.2.1.3 | E361A |
inactive mutant enzyme |
652285 |
6.2.1.3 | F319A |
site-directed mutagenesis |
714605 |
6.2.1.3 | F319W |
site-directed mutagenesis |
714605 |
6.2.1.3 | F319Y |
site-directed mutagenesis |
714605 |
6.2.1.3 | G216A |
the ratio of turnover number to Km-value for the reaction with oleate is 32.7% of the wild-type ratio |
652285 |
6.2.1.3 | G219A |
the ratio of turnover number to Km-value for the reaction with oleate is 1.3fold higher than the wild-type ratio |
652285 |