EC Number |
Protein Variants |
Reference |
---|
5.6.1.5 | E271Q |
Walker B mutant |
751651 |
5.6.1.5 | E365A/D367A |
mutant constructed for interaction studies between the C-terminal domain of Rpt3 and Nas6, the complex is completely disrupted for this mutant |
684857 |
5.6.1.5 | G158E |
the mutation impairs the light-specific hypocotyl elongation response elicited by class I S(1)-b-methyl-a,b-diaminopropionic acid, the mutated amino acid site resides within the full AAA-ATPase domain |
700778 |
5.6.1.5 | G360A/G387A |
site-directed mutagenesis, the Rpt6 mutant reduces the Rpn14 association with proteasome base and disrupts holoenzyme formation in vitro |
735289 |
5.6.1.5 | K229R |
lethal mutation |
654902 |
5.6.1.5 | K229R |
lysine residue is essentiel for ATP hydrolysis, mutation is lethal |
656773 |
5.6.1.5 | K397A/R399A |
mutant constructed for interaction studies between the C-terminal domain of Rpt3 and Nas6, the complex is completely disrupted for this mutant |
684857 |
5.6.1.5 | M87A |
the mutant shows almost wild type activity |
-, 751668 |
5.6.1.5 | more |
conditional knockdown yeast strains for each Rpt subunit. Since all Rpt subunits are lethal to almost all species, including yeast and higher species, rescue experiments using individual Rpt subunits in conditional knockdown strains for each Rpt subunit are conducted to analyze the functional compensation of exogenic mutant Rpt subunits in cells in which the expression of endogenous Rpt subunits is conditionally suppressed. Mouse Rpt subunits can be substituted for yeast Rpt subunits |
735031 |
5.6.1.5 | more |
disruption of nucleotide binding to an Rpt subunit by mutation in the Walker A motif, mutation in the Walker B motif, deletion of the HbYX motif, C-terminal deletions in Rpts not possessing the HbYX motif |
734207 |