EC Number   |
Protein Variants |
Reference |
|---|
   5.4.99.B22 | D112A |
inactive mutant enzyme |
682834 |
| 5.4.99.B22 | D146A |
site-directed mutagenesis of truncated enzyme mutant DELTAhPus1p, differences between structures of DELTAhPus1p and DELTAhPus1pD146A are limited to external, poorly conserved loops, which confirm the flexibility of these regions |
749148 |
| 5.4.99.B22 | more |
generation of a truncated hPus1p version encompassing residues 83-394, i.e. DELTAhPus1p. The deleted N- and C-termini are not essential for either the RNA binding or the activity of the hPus1p enzyme |
749148 |
| 5.4.99.B22 | more |
generation of gene deletion mutant DELTApus1. which shows a U2 snRNA modification pattern identical to its parental wild-type strain ED666 |
-, 749279 |
| 5.4.99.B22 | more |
generation of Pus1 knockout mice |
749279 |
| 5.4.99.B22 | more |
SNORA71 is expressed in an enzyme knockout mutant yeast strain, but do not restore pseudouridylation of the endogenous yeast U2 snRNA |
749279 |
| 5.4.99.B22 | R116A |
the mutant exhibits some activity with the yeast pre-tRNAIle substrate. Low activity with the mouse tRNAMet substrate |
695106 |
| 5.4.99.B22 | R116C |
the activity of the mutant enzyme with the yeast pre-tRNAIle substrate is approximately one-third that of the wild-type levels. No activity with the mouse tRNAMet substrate |
695106 |
| 5.4.99.B22 | R116E |
the mutant is inactive with with the yeast pre-tRNAIle substrate. No activity with the mouse tRNAMet substrate |
695106 |
| 5.4.99.B22 | R116G |
the mutant exhibits some activity with the yeast pre-tRNAIle substrate. No activity with the mouse tRNAMet substrate |
695106 |
