EC Number   |
Protein Variants |
Reference |
|---|
   5.4.99.28 | C117A |
kcat is 1.4fold lower than wild-type value, KM is 3.9fold higher than wild-type value |
648867 |
| 5.4.99.28 | C117A/A128A |
kcat is 1.3fold lower than wild-type value, KM is 1.5fold higher than wild-type value |
648867 |
| 5.4.99.28 | C128A |
kcat is 1.5fold higher than wild-type value, KM is 1.5fold higher than wild-type value |
648867 |
| 5.4.99.28 | D112A |
mutant does not pseudouridine32 in formation in cytoplasmic tRNAGly of the DELTArib2/pus8/DELTApus9 strain |
712356 |
| 5.4.99.28 | more |
Pus9P lacking the 23 N-terminal amino acids is inactive |
712356 |
| 5.4.99.28 | more |
substitution of cysteine for amino acids with nonnucleophilic side chains does not significantly alter the catalytic activity |
648867 |
| 5.4.99.28 | P29G |
kcat-value is 1.6fold lower than wild-type value. KM-value is 2.9fold higher than wild-type value |
702203 |
| 5.4.99.28 | P29L |
kcat-value is 1.9fold lower than wild-type value. KM-value is 4.2fold higher than wild-type value |
702203 |
