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EC Number	Protein Variants	Commentary	Reference
5.4.99.27	A78R/N79H	mutation has no positive effect on activity at 80°C on Pyrococcus abyssi tRNAAsp(GUA)	694463
5.4.99.27	E31D	27% of wild-type activity	701924
5.4.99.27	E31Q	no activity	701924
5.4.99.27	E31Q/F131Y	no activity	701924
5.4.99.27	F131Y	68% of wild-type activity	701924
5.4.99.27	I27K	mutation increases activity at 80°C on Pyrococcus abyssi tRNAAsp(GUA) 1.6fold	694463
5.4.99.27	K19I	mutation decreases the activity at position 13 in the Pyrococcus abyssi tRNAAsp at 55°C. At 80°C the mutation has almost no negative effect on its activity towards the Pyrococcus abyssi tRNATyr(GUA)	694463
5.4.99.27	K19I/R78A/H79N	mutation completely abolishes the activity at position 13 in the Pyrococcus abyssi tRNAAsp at 55°C. At 80°C the mutation nearly abolishes activity towards the Pyrococcus abyssi tRNATyr(GUA)	694463
5.4.99.27	K79L	5% of wild-type activity	701924
5.4.99.27	K79L	no activity	701924

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Release 2023.1 (January 2023)