EC Number   |
Protein Variants |
Reference |
|---|
   5.4.99.25 | C106A/C109A |
decrease in tRNA pseudouridine54 synthase activity, no decrease in tRNA pseudouridine55 synthase activity |
728787 |
| 5.4.99.25 | C174A |
kcat is 1.3fold higher than wild-type value, KM is 1.1fold lower than wild-type value |
648867 |
| 5.4.99.25 | C193V |
kcat is 1.5fold higher than wild-type value, KM is 1.5fold higher than wild-type value |
648867 |
| 5.4.99.25 | C58A |
kcat is 2.2fold higher than wild-type value, KM is 1.2fold higher than wild-type value |
648867 |
| 5.4.99.25 | C58A/C174A/C193A |
kcat is 1.1fold lower than wild-type value, KM is 1.2fold lower than wild-type value |
648867 |
| 5.4.99.25 | D275A |
the mutant shows no tRNA pseudouridine54 synthase activity and no tRNA pseudouridine55 synthase activity |
-, 728787 |
| 5.4.99.25 | D277A |
decrease in tRNA pseudouridine54 synthase activity and low decrease in tRNA pseudouridine55 synthase activity |
-, 728787 |
| 5.4.99.25 | D48N |
inactive mutant enzyme |
663343 |
| 5.4.99.25 | D90A |
dissociation constant: 0.4 microM (wild-type: 0.7 microM), rate of pseudouridine formation compared to wild-type: 500fold decreased, rate of tRNA binding (kapp2): 2/sec (wild-type: 4.2/sec) |
730510 |
| 5.4.99.25 | D90E |
dissociation constant: 0.4 microM (wild-type: 0.7 microM), rate of pseudouridine formation compared to wild-type: 30fold decreased, rate of tRNA binding (kapp2): 1.5/sec (wild-type: 4.2/sec) |
730510 |
